ArticleProceedings of the National Academy of Sciences of the United States of America2010
A potential role for initiator-tRNA in pre-mRNA splicing regulation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- White matter disorders at the intersection of transcription, RNA processing and translation.Nature reviews. Neurology · 2026Review
- Noncoding RNAs: The Not So "Junk" DNA Is the Blueprint of the Transcriptional Landscape of the RNA World.Experientia supplementum (2012) · 2026Review
- Identification of RNA binding proteins that mediate a quality control mechanism of splicing.bioRxiv : the preprint server for biology · 2025Article
- tRNA and tsRNA: From Heterogeneity to Multifaceted Regulators.Biomolecules · 2024Review
- Protein translation: biological processes and therapeutic strategies for human diseases.Signal transduction and targeted therapy · 2024Review
- A Quality Control Mechanism of Splice Site Selection Abrogated under Stress and in Cancer.Cancers · 2022Review
- A novel role for nucleolin in splice site selection.RNA biology · 2022Article
- Noncanonical Roles of tRNAs: tRNA Fragments and Beyond.Annual review of genetics · 2020Review
- Article
- Structural studies of the endogenous spliceosome - The supraspliceosome.Methods (San Diego, Calif.) · 2017Review
- A genetically encoded fluorescent tRNA is active in live-cell protein synthesis.Nucleic acids research · 2017Article
- Heat shock activates splicing at latent alternative 5' splice sites in nematodes.Nucleus (Austin, Tex.) · 2015Article
- The Supraspliceosome - A Multi-Task Machine for Regulated Pre-mRNA Processing in the Cell Nucleus.Computational and structural biotechnology journal · 2014Review
- Function of alternative splicing.Gene · 2013Review
- Genome-wide activation of latent donor splice sites in stress and disease.Nucleic acids research · 2012Article
- Solution nuclear magnetic resonance analyses of the anticodon arms of proteinogenic and nonproteinogenic tRNA(Gly).Biochemistry · 2012Article
- Cotranscriptional effect of a premature termination codon revealed by live-cell imaging.RNA (New York, N.Y.) · 2011Article
- Apoptotic regulation and tRNA.Protein & cell · 2010Review
- Premature Termination Codons Are Recognized in the Nucleus in A Reading-Frame Dependent Manner.Cell discoveryArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The translation initiator-tRNA plays a crucial role in the initiation of protein synthesis in both prokaryotic and eukaryotic cells, by employing specific base pairing between its anticodon triplet CAU and the general initiation codon AUG in the mRNA. Here we show that the initiator-tRNA may also act, in a manner that is independent of its role in protein translation, as a pre-mRNA splicing regulator. Specifically, we show that alternative splicing events that are induced by mutations in the translation initiation AUG codon can be suppressed by expressing initiator-tRNA constructs carrying anticodon mutations that compensate for the AUG mutations. These mutated initiator-tRNAs appeared to be uncharged with an amino acid. Our results imply that recognition of the initiation AUG sequence by the anticodon triplet of initiator-tRNA in its unloaded state plays a role in quality control of splicing in the cell nucleus by a yet unresolved mechanism. Identifying the initiator-tRNA as a transacting splicing regulator suggests a novel involvement of this molecule in splicing regulation and provides a critical step toward deciphering this intriguing mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.