ArticleProceedings of the National Academy of Sciences of the United States of America2010
Structure of the Qbeta replicase, an RNA-dependent RNA polymerase consisting of viral and host proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 71 citations in OpenAlex.
- Article
- Diverse bacterial pattern recognition receptors sense the conserved phage proteome.bioRxiv : the preprint server for biology · 2026Article
- Impact of simulated microgravity in short-term evolution of an RNA bacteriophage.Frontiers in microbiology · 2025Article
- Evolutionary Adaptation of an RNA Bacteriophage to Repeated Freezing and Thawing Cycles.International journal of molecular sciences · 2024Article
- Review
- In vitro characterisation of the MS2 RNA polymerase complex reveals host factors that modulate emesviral replicase activity.Communications biology · 2022Article
- RdRp (RNA-dependent RNA polymerase): A key target providing anti-virals for the management of various viral diseases.Journal of molecular structure · 2022Review
- Relaxed Substrate Specificity in Qβ Replicase through Long-Term In Vitro Evolution.Life (Basel, Switzerland) · 2021Article
- NMR structure of the Vibrio vulnificus ribosomal protein S1 domains D3 and D4 provides insights into molecular recognition of single-stranded RNAs.Nucleic acids research · 2021Article
- Within and Beyond the Nucleotide Addition Cycle of Viral RNA-dependent RNA Polymerases.Frontiers in molecular biosciences · 2021Article
- Discovery of divided RdRp sequences and a hitherto unknown genomic complexity in fungal viruses.Virus evolution · 2021Article
- Applications of phage-derived RNA-based technologies in synthetic biology.Synthetic and systems biotechnology · 2020Review
- Rapid de novo evolution of lysis genes in single-stranded RNA phages.Nature communications · 2020Article
- Cell-free expression of RNA encoded genes using MS2 replicase.Nucleic acids research · 2019Article
- Evolutionary Dynamics in the RNA Bacteriophage Qβ Depends on the Pattern of Change in Selective Pressures.Pathogens (Basel, Switzerland) · 2019Article
- Reverse Genetic Systems forMethods and protocols · 2019Article
- A Structure-Function Diversity Survey of the RNA-Dependent RNA Polymerases From the Positive-Strand RNA Viruses.Frontiers in microbiology · 2019Review
- Function of the RNA Coliphage Qβ Proteins in Medical In Vitro Evolution.Methods and protocols · 2018Review
- Review
- Metagenomics reshapes the concepts of RNA virus evolution by revealing extensive horizontal virus transfer.Virus research · 2018Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The RNA-dependent RNA polymerase core complex formed upon infection of Escherichia coli by the bacteriophage Qbeta is composed of the viral catalytic beta-subunit as well as the host translation elongation factors EF-Tu and EF-Ts, which are required for initiation of RNA replication. We have determined the crystal structure of the complex between the beta-subunit and the two host proteins to 2.5-A resolution. Whereas the basic catalytic machinery in the viral subunit appears similar to other RNA-dependent RNA polymerases, a unique C-terminal region of the beta-subunit engages in extensive interactions with EF-Tu and may contribute to the separation of the transient duplex formed between the template and the nascent product to allow exponential amplification of the phage genome. The evolution of resistance by the host appears to be impaired because of the interactions of the beta-subunit with parts of EF-Tu essential in recognition of aminoacyl-tRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.