ArticleProceedings of the National Academy of Sciences of the United States of America2010
Ribosome recycling step in yeast cytoplasmic protein synthesis is catalyzed by eEF3 and ATP.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Yeast elongation factor homolog New1 protects a subset of mRNAs from degradation by no-go decay.Nucleic acids research · 2026Article
- Exploring the role and mechanisms of theMycology · 2025Article
- The geneFrontiers in microbiology · 2024Article
- Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis.Nature communications · 2022Article
- ABC-F translation factors: from antibiotic resistance to immune response.FEBS letters · 2021Review
- Eukaryotic Elongation Factor 3 ProtectsGenes · 2020Article
- A Quick Guide to Small-Molecule Inhibitors of Eukaryotic Protein Synthesis.Biochemistry. Biokhimiia · 2020Review
- Biochemical characterization of the mouse ABCF3 protein, a partner of the flavivirus-resistance protein OAS1B.The Journal of biological chemistry · 2019Article
- A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling.Nucleic acids research · 2019Article
- ABCF ATPases Involved in Protein Synthesis, Ribosome Assembly and Antibiotic Resistance: Structural and Functional Diversification across the Tree of Life.Journal of molecular biology · 2019Article
- Ribosome profiling analysis of eEF3-depleted Saccharomyces cerevisiae.Scientific reports · 2019Article
- Identification of a 57S translation complex containing closed-loop factors and the 60S ribosome subunit.Scientific reports · 2018Article
- Transcriptomic analysis ofF1000Research · 2017Article
- Article
- Evidence That Antibiotics Bind to Human Mitochondrial Ribosomal RNA Has Implications for Aminoglycoside Toxicity.The Journal of biological chemistry · 2015Article
- Transcriptomics of cryophilic Saccharomyces kudriavzevii reveals the key role of gene translation efficiency in cold stress adaptations.BMC genomics · 2014Article
- The ABC-F protein EttA gates ribosome entry into the translation elongation cycle.Nature structural & molecular biology · 2014Article
- The ABCs of the ribosome.Nature structural & molecular biology · 2014Article
- Possible steps of complete disassembly of post-termination complex by yeast eEF3 deduced from inhibition by translocation inhibitors.Nucleic acids research · 2013Article
- Article
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
After each round of protein biosynthesis, the posttermination complex (PoTC) consisting of a ribosome, mRNA, and tRNA must be disassembled into its components for a new round of translation. Here, we show that a Saccharomyces cerevisiae model PoTC was disassembled by ATP and eukaryotic elongation factor 3 (eEF3). GTP or ITP functioned with less efficiency and adenosine 5gamma'-(beta,gamma-imido)triphosphate did not function at all. The k(cat) of eEF3 was 1.12 min(-1), which is comparable to that of the in vitro initiation step. The disassembly reaction was inhibited by aminoglycosides and cycloheximide. The subunits formed from the yeast model PoTC remained separated under ionic conditions close to those existing in vivo, suggesting that they are ready to enter the initiation process. Based on our experimental techniques used in this paper, the release of mRNA and tRNA and ribosome dissociation took place simultaneously. No 40S*mRNA complex was observed, indicating that eEF3 action promotes ribosome recycling, not reinitiation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.