ArticleBMC microbiology2010
Telonemia-specific environmental 18S rDNA PCR reveals unknown diversity and multiple marine-freshwater colonizations.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Phylogenomic Analysis of Deep-Branching Telonemid.Genome biology and evolution · 2025Article
- DNA Metabarcoding as a Tool to Study Plankton Responses to Warming and Salinity Change in Mesocosms.Ecology and evolution · 2025Article
- Phylogenetic position and mitochondrial genome evolution of "orphan" eukaryotic lineages.iScience · 2025Article
- Integrating depth-dependent protist dynamics and microbial interactions in spring succession of a freshwater reservoir.Environmental microbiome · 2024Article
- Diverse winter communities and biogeochemical cycling potential in the under-ice microbial plankton of a subarctic river-to-sea continuum.Microbiology spectrum · 2024Article
- VESPA: an optimized protocol for accurate metabarcoding-based characterization of vertebrate eukaryotic endosymbiont and parasite assemblages.Nature communications · 2024Article
- Global freshwater distribution of Telonemia protists.The ISME journal · 2024Article
- Global patterns and rates of habitat transitions across the eukaryotic tree of life.Nature ecology & evolution · 2022Article
- On the origin of TSAR: morphology, diversity and phylogeny of Telonemia.Open biology · 2022Article
- CARD-FISH in the Sequencing Era: Opening a New Universe of Protistan Ecology.Frontiers in microbiology · 2021Review
- Unraveling the Diversity of Eukaryotic Microplankton in a Large and Deep Perialpine Lake Using a High Throughput Sequencing Approach.Frontiers in microbiology · 2020Article
- New Phylogenomic Analysis of the Enigmatic Phylum Telonemia Further Resolves the Eukaryote Tree of Life.Molecular biology and evolution · 2019Article
- Annual Protist Community Dynamics in a Freshwater Ecosystem Undergoing Contrasted Climatic Conditions: The Saint-Charles River (Canada).Frontiers in microbiology · 2019Article
- Enigmatic Diphyllatea eukaryotes: culturing and targeted PacBio RS amplicon sequencing reveals a higher order taxonomic diversity and global distribution.BMC evolutionary biology · 2018Article
- Untangling the early diversification of eukaryotes: a phylogenomic study of the evolutionary origins of Centrohelida, Haptophyta and Cryptista.Proceedings. Biological sciences · 2016Article
- Strong Seasonality of Marine Microbial Eukaryotes in a High-Arctic Fjord (Isfjorden, in West Spitsbergen, Norway).Applied and environmental microbiology · 2016Article
- Biogeography of heterotrophic flagellate populations indicates the presence of generalist and specialist taxa in the Arctic Ocean.Applied and environmental microbiology · 2015Article
- Communities of microbial eukaryotes in the mammalian gut within the context of environmental eukaryotic diversity.Frontiers in microbiology · 2014Article
- The new micro-kingdoms of eukaryotes.BMC biology · 2013Review
- Molecular detection, quantification, and diversity evaluation of microalgae.Marine biotechnology (New York, N.Y.) · 2012Review
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Authors and funding
5 authors.
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Abstract
backgroundRecent surveys of eukaryote 18S rDNA diversity in marine habitats have uncovered worldwide distribution of the heterotrophic eukaryote phylum Telonemia. Here we investigate the diversity and geographic distribution of Telonemia sequences by in-depth sequencing of several new 18S rDNA clone libraries from both marine and freshwater sites by using a Telonemia-specific PCR strategy.
resultsIn contrast to earlier studies that have employed eukaryote-wide PCR design, we identified a large and unknown diversity of phylotypes and the first rigorous evidence for several freshwater species, altogether comprising 91 unique sequences. Phylogenies of these and publicly available sequences showed 20 statistically supported sub-clades as well as several solitary phylotypes with no clear phylogenetic affiliation. Most of these sub-clades were composed of phylotypes from different geographic regions.
conclusionsBy using specific PCR primers we reveal a much larger diversity of Telonemia from environmental samples than previously uncovered by eukaryote-wide primers. The new data substantially diminish the geographic structuring of clades identified in earlier studies. Nevertheless, since these clades comprise several distinct phylotypes we cannot exclude endemicity at species level. We identified two freshwater clades and a few solitary phylotypes, implying that Telonemia have colonized freshwater habitats and adapted to the different environmental and ecological conditions at independent occasions.
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