ArticlePlant cell reports2010
Cucumber mosaic virus 2b protein inhibits RNA silencing pathways in green alga Chlamydomonas reinhardtii.
Article in Plant cell reports, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 11 citations in OpenAlex.
- CRISPR/Cas9-induced knockout and knock-in mutations in Chlamydomonas reinhardtii.Scientific reports · 2016Article
- Did silencing suppression counter-defensive strategy contribute to origin and evolution of the triple gene block coding for plant virus movement proteins?Frontiers in plant science · 2012Article
- RNA-mediated silencing in Algae: biological roles and tools for analysis of gene function.Eukaryotic cell · 2011Review
- Dicer functions in aquatic species.Journal of amino acids · 2011Article
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The functions of RNA silencing are repression of endogenous gene expression and antiviral defense in plants and animals. Cucumber mosaic virus 2b (CMV2b) is a suppressor of RNA silencing in higher plants. In the present study, we evaluated the RNA silencing suppressor activity of CMV2b in Chlamydomonas reinhardtii. Before transformation, we modified CMV2b codons to increase the GC content for optimal expression in C. reinhardtii. Inhibition of Maa7 silencing was detected in CMV2b-expressing Maa7-IR44 strains, indicating that CMV2b suppressed siRNA pathways in C. reinhardtii as in higher plants. In addition, mRNA expression targeted for cleavage by miRNA was significantly higher in CMV2b-expressing strains, but increased accumulation of miRNA was not detected. These results indicate that the suppression of miRNA pathways is mediated by CMV2b in C. reinhardtii. Interestingly, expression of both Argonaute 1 (AGO1) and Dicer-like 1 (DCL1), regulated by a bidirectional promoter, was reduced in CMV2b-expressing strains, suggesting that CMV2b may affect transcription factors involved in RNA silencing pathways. Furthermore, reduction of AGO2 and AGO3 expression was detected in CMV2b-expressing strains. Taken together, our results demonstrate that CMV2b may suppress both siRNA and miRNA pathways, and also impair AGOs and DCL1 expression in C. reinhardtii.
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