ArticlePloS one2010
Functional characterization of the Frost gene in Drosophila melanogaster: importance for recovery from chill coma.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 54 citations in OpenAlex.
- Fitness Analysis and Transcriptome Profiling Following Repeated Mild Heat Stress of Varying Frequency inBiology · 2021Article
- First transcriptome profiling of D. melanogaster after development in a deep underground low radiation background laboratory.PloS one · 2021Article
- Climate stress resistance in male Queensland fruit fly varies among populations of diverse geographic origins and changes during domestication.BMC genetics · 2020Article
- Integrating GWAS and Transcriptomics to Identify the Molecular Underpinnings of Thermal Stress Responses inFrontiers in genetics · 2020Article
- A transcriptional and functional analysis of heat hardening in two invasive fruit fly species,Evolutionary applications · 2019Article
- Parental obesity leads to metabolic changes in the F2 generation inMolecular metabolism · 2017Article
- Large scale phosphoprotein profiling to explore Drosophila cold acclimation regulatory mechanisms.Scientific reports · 2017Article
- Effects of cold-acclimation on gene expression in Fall field cricket (Gryllus pennsylvanicus) ionoregulatory tissues.BMC genomics · 2017Article
- Canalization of gene expression is a major signature of regulatory cold adaptation in temperate Drosophila melanogaster.BMC genomics · 2016Article
- The Drosophila melanogaster Muc68E Mucin Gene Influences Adult Size, Starvation Tolerance, and Cold Recovery.G3 (Bethesda, Md.) · 2016Article
- Cold acclimation wholly reorganizes the Drosophila melanogaster transcriptome and metabolome.Scientific reports · 2016Article
- Slowed aging during reproductive dormancy is reflected in genome-wide transcriptome changes in Drosophila melanogaster.BMC genomics · 2016Article
- Long-term recovery from acute cold shock in Caenorhabditis elegans.BMC cell biology · 2016Article
- Insect capa neuropeptides impact desiccation and cold tolerance.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Role of high-fat diet in stress response of Drosophila.PloS one · 2012Article
- Infection-related declines in chill coma recovery and negative geotaxis in Drosophila melanogaster.PloS one · 2012Article
- Rapid growth reduces cold resistance: evidence from latitudinal variation in growth rate, cold resistance and stress proteins.PloS one · 2011Article
- Interactions between Controlled Atmospheres and Low Temperature Tolerance: A Review of Biochemical Mechanisms.Frontiers in physiology · 2011Article
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlmost all animals, including insects, need to adapt to temperature fluctuations. The molecular basis of thermal adaptation is not well understood, although a number of candidate genes have been proposed. However, a functional link between candidate genes and thermal tolerance has rarely been established. The gene Frost (Fst) was first discovered when Drosophila flies were exposed to cold stress, but the biological function(s) of Fst has so far not been characterized. Because Fst is up-regulated after a cold stress, we tested whether it was essential for chill-coma recovery. METHODOLOGY/PRINCIPAL
findingsA marked increase in Fst expression was detected (by RT-PCR) during recovery from cold stress, peaking at 42-fold after 2 h. The GAL4/UAS system was used to knock down expression of Fst and recovery ability was assessed in transgenic adults following 12 h of chill coma at 0 degrees C. The ability to recover from cold stress (short-, medium- and long-term) was significantly altered in the transgenic adults that had Fst silenced. These findings show that Fst plays an essential role in the recovery from chill coma in both males and females. CONCLUSIONS/SIGNIFICANCE: The Frost gene is essential for cold tolerance in Drosophila melanogaster and may play an important role in thermal adaptation.
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