ArticleMolecular systems biology2010
Clustering phenotype populations by genome-wide RNAi and multiparametric imaging.
Article in Molecular systems biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
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Who cites it
90 citing papers in PubMed.
- A pooled Cell Painting CRISPR screening platform enables de novo inference of gene function by self-supervised deep learning.Nature communications · 2025Article
- The DNA replication machinery transmits dual signals to prevent unscheduled licensing and execution of centrosome duplication.Nature communications · 2025Article
- Capturing cell heterogeneity in representations of cell populations for image-based profiling using contrastive learning.PLoS computational biology · 2024Article
- Capturing cell heterogeneity in representations of cell populations for image-based profiling using contrastive learning.bioRxiv : the preprint server for biology · 2024Article
- DONSON is required for CMG helicase assembly in the mammalian cell cycle.EMBO reports · 2023Article
- The expanding genetic and clinical landscape associated with Meier-Gorlin syndrome.European journal of human genetics : EJHG · 2023Review
- Single object profiles regression analysis (SOPRA): a novel method for analyzing high-content cell-based screens.BMC bioinformatics · 2022Article
- Neurodevelopmental copy-number variants: A roadmap to improving outcomes by uniting patient advocates, researchers, and clinicians for collective impact.American journal of human genetics · 2022Review
- Allele-specific endogenous tagging and quantitative analysis of β-catenin in colorectal cancer cells.eLife · 2022Article
- Cell-TypeAnalyzer: A flexible Fiji/ImageJ plugin to classify cells according to user-defined criteria.Biological imaging · 2022Article
- Arrayed CRISPR reveals genetic regulators of tau aggregation, autophagy and mitochondria in Alzheimer's disease model.Scientific reports · 2021Article
- Downstream Neighbor of SON (DONSON) Expression Is Enhanced in Phenotypically Aggressive Prostate Cancers.Cancers · 2020Article
- Downstream neighbor of SON (DONSON) is associated with unfavorable survival across diverse cancers with oncogenic properties in clear cell renal cell carcinoma.Translational oncology · 2020Article
- Machine learning-aided quantification of antibody-based cancer immunotherapy by natural killer cells in microfluidic droplets.Lab on a chip · 2020Article
- Linked-read genome sequencing identifies biallelic pathogenic variants inJournal of medical genetics · 2020Article
- KCML: a machine-learning framework for inference of multi-scale gene functions from genetic perturbation screens.Molecular systems biology · 2020Article
- High-Content Image-Based Single-Cell Phenotypic Analysis for the Testicular Toxicity Prediction Induced by Bisphenol A and Its Analogs Bisphenol S, Bisphenol AF, and Tetrabromobisphenol A in a Three-Dimensional Testicular Cell Co-culture Model.Toxicological sciences : an official journal of the Society of Toxicology · 2020Article
- Recent insights into the genotype-phenotype relationship from massively parallel genetic assays.Evolutionary applications · 2019Review
- Assembly of a parts list of the human mitotic cell cycle machinery.Journal of molecular cell biology · 2019Article
- Capturing Single-Cell Phenotypic Variation via Unsupervised Representation Learning.Proceedings of machine learning research · 2019Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genetic screens for phenotypic similarity have made key contributions to associating genes with biological processes. With RNA interference (RNAi), highly parallel phenotyping of loss-of-function effects in cells has become feasible. One of the current challenges however is the computational categorization of visual phenotypes and the prediction of biological function and processes. In this study, we describe a combined computational and experimental approach to discover novel gene functions and explore functional relationships. We performed a genome-wide RNAi screen in human cells and used quantitative descriptors derived from high-throughput imaging to generate multiparametric phenotypic profiles. We show that profiles predicted functions of genes by phenotypic similarity. Specifically, we examined several candidates including the largely uncharacterized gene DONSON, which shared phenotype similarity with known factors of DNA damage response (DDR) and genomic integrity. Experimental evidence supports that DONSON is a novel centrosomal protein required for DDR signalling and genomic integrity. Multiparametric phenotyping by automated imaging and computational annotation is a powerful method for functional discovery and mapping the landscape of phenotypic responses to cellular perturbations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.