ArticleJournal of cell science2010
The yeast CLC protein counteracts vesicular acidification during iron starvation.
Article in Journal of cell science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 46 citations in OpenAlex.
- RcAlb-PepII Perturbs the Proteomic Profile ofMicroorganisms · 2026Article
- The Ferroxidase-Permease System for Transport of Iron Across Membranes: From Yeast to Humans.International journal of molecular sciences · 2025Review
- In Vivo Imaging with Genetically Encoded Redox Biosensors.International journal of molecular sciences · 2020Review
- Uncoupling endosomal CLC chloride/proton exchange causes severe neurodegeneration.The EMBO journal · 2020Article
- A new pH sensor localized in the Golgi apparatus of Saccharomyces cerevisiae reveals unexpected roles of Vph1p and Stv1p isoforms.Scientific reports · 2020Article
- Activation leads to a significant shift in the intracellular redox homeostasis of neutrophil-like cells.Redox biology · 2020Article
- The signaling lipid phosphatidylinositol-3,5-bisphosphate targets plant CLC-a anion/HEMBO reports · 2017Article
- Regulators of Lysosome Function and Dynamics inG3 (Bethesda, Md.) · 2017Article
- Identification and characterization of the three members of the CLC family of anion transport proteins in Trypanosoma brucei.PloS one · 2017Article
- Copper trafficking to the secretory pathway.Metallomics : integrated biometal science · 2016Review
- Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase.The Journal of biological chemistry · 2016Article
- Proton Transport and pH Control in Fungi.Advances in experimental medicine and biology · 2016Review
- Kinetic control by limiting glutaredoxin amounts enables thiol oxidation in the reducing mitochondrial intermembrane space.Molecular biology of the cell · 2015Article
- Ist2 in the yeast cortical endoplasmic reticulum promotes trafficking of the amino acid transporter Bap2 to the plasma membrane.PloS one · 2014Article
- Measurement of vacuolar and cytosolic pH in vivo in yeast cell suspensions.Journal of visualized experiments : JoVE · 2013Article
- Regulation of cation balance in Saccharomyces cerevisiae.Genetics · 2013Review
- Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis.Nature chemical biology · 2013Article
- The GEF1 proton-chloride exchanger affects tombusvirus replication via regulation of copper metabolism in yeast.Journal of virology · 2013Article
- Real-time imaging of the intracellular glutathione redox potential in the malaria parasite Plasmodium falciparum.PLoS pathogens · 2013Article
- OnGuard, a computational platform for quantitative kinetic modeling of guard cell physiology.Plant physiology · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Ion gradients across intracellular membranes contribute to the physicochemical environment inside compartments. CLC anion transport proteins that localise to intracellular organelles are anion-proton exchangers involved in anion sequestration or vesicular acidification. By homology, the only CLC protein of Saccharomyces cerevisiae, Gef1, belongs to this family of intracellular exchangers. Gef1 localises to the late Golgi and prevacuole and is essential in conditions of iron limitation. In the absence of Gef1, a multicopper oxidase involved in iron uptake, Fet3, fails to acquire copper ion cofactors. The precise role of the exchanger in this physiological context is unknown. Here, we show that the Gef1-containing compartment is adjusted to a more alkaline pH under iron limitation. This depends on the antiport function of Gef1, because an uncoupled mutant of Gef1 (E230A) results in the acidification of the lumen and fails to support Fet3 maturation. Furthermore, we found that Gef1 antiport activity correlates with marked effects on cellular glutathione homeostasis, raising the possibility that the effect of Gef1 on Fet3 copper loading is related to the control of compartmental glutathione concentration or redox status. Mutational inactivation of a conserved ATP-binding site in the cytosolic cystathione beta-synthetase domain of Gef1 (D732A) suggests that Gef1 activity is regulated by energy metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.