ArticleNucleic acids research2010
Zinc-finger nuclease-driven targeted integration into mammalian genomes using donors with limited chromosomal homology.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 95 papers.
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Who cites it
95 citing papers in PubMed, 192 citations in OpenAlex.
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- Low Efficiency of Homology-Independent Targeted Integration for CRISPR/Cas9 Correction in the Vicinity of theInternational journal of molecular sciences · 2025Article
- CRISPR and gene editing technologies for bleeding disorders.Therapeutic advances in hematology · 2025Review
- Outlook on genome editing application to cattle.Journal of veterinary science · 2024Review
- Efficient protein tagging and cis-regulatory element engineering via precise and directional oligonucleotide-based targeted insertion in plants.The Plant cell · 2023Article
- Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors.Molecular biology reports · 2023Article
- Chimeric RNA: DNA TracrRNA Improves Homology-Directed RepairThe CRISPR journal · 2022Article
- CRISPR Editing Enables Consequential Tag-Activated MicroRNA-Mediated Endogene Deactivation.International journal of molecular sciences · 2022Article
- A HIT-trapping strategy for rapid generation of reversible and conditional alleles using a universal donor.Genome research · 2021Article
- A homology independent sequence replacement strategy in human cells using a CRISPR nuclease.Open biology · 2021Article
- Live cell imaging and proteomic profiling of endogenous NEAT1 lncRNA by CRISPR/Cas9-mediated knock-in.Protein & cell · 2020Article
- CRISPR-Cas12a-assisted PCR tagging of mammalian genes.The Journal of cell biology · 2020Article
- CRISPR/Cas9-Mediated Integration of Large Transgene into PigG3 (Bethesda, Md.) · 2020Article
- Generation of a conditional mutant knock-in under the control of the natural promoter using CRISPR-Cas9 and Cre-Lox systems.PloS one · 2020Article
- Enhancing gene editing specificity by attenuating DNA cleavage kinetics.Nature biotechnology · 2019Article
- Zinc finger nuclease-mediated targeting of multiple transgenes to an endogenous soybean genomic locus via non-homologous end joining.Plant biotechnology journal · 2019Article
- CRISPR-Cas: Converting A Bacterial Defence Mechanism into A State-of-the-Art Genetic Manipulation Tool.Antibiotics (Basel, Switzerland) · 2019Review
- Clinical trials of dual-target CAR T cells, donor-derived CAR T cells, and universal CAR T cells for acute lymphoid leukemia.Journal of hematology & oncology · 2019Review
- Plant DNA Repair Pathways and Their Applications in Genome Engineering.Methods in molecular biology (Clifton, N.J.) · 2019Review
- Zinc finger nuclease-mediated precision genome editing of an endogenous gene in hexaploid bread wheat (Triticum aestivum) using a DNA repair template.Plant biotechnology journal · 2018Article
35 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
15 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously demonstrated high-frequency, targeted DNA addition mediated by the homology-directed DNA repair pathway. This method uses a zinc-finger nuclease (ZFN) to create a site-specific double-strand break (DSB) that facilitates copying of genetic information into the chromosome from an exogenous donor molecule. Such donors typically contain two approximately 750 bp regions of chromosomal sequence required for homology-directed DNA repair. Here, we demonstrate that easily-generated linear donors with extremely short (50 bp) homology regions drive transgene integration into 5-10% of chromosomes. Moreover, we measure the overhangs produced by ZFN cleavage and find that oligonucleotide donors with single-stranded 5' overhangs complementary to those made by ZFNs are efficiently ligated in vivo to the DSB. Greater than 10% of all chromosomes directly incorporate this exogenous DNA via a process that is dependent upon and guided by complementary 5' overhangs on the donor DNA. Finally, we extend this non-homologous end-joining (NHEJ)-based technique by directly inserting donor DNA comprising recombinase sites into large deletions created by the simultaneous action of two separate ZFN pairs. Up to 50% of deletions contained a donor insertion. Targeted DNA addition via NHEJ complements our homology-directed targeted integration approaches, adding versatility to the manipulation of mammalian genomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.