ArticleThe Journal of biological chemistry2010
A nucleotide state-sensing region on actin.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Spa2 remodels ADP-actin via molecular condensation under glucose starvation.Nature communications · 2024Article
- Structural Significance of His73 in F-Actin Dynamics: Insights from Ab Initio Study.International journal of molecular sciences · 2022Article
- Article
- Bound nucleotide can control the dynamic architecture of monomeric actin.Nature structural & molecular biology · 2022Article
- Article
- Oligomerization Affects the Ability of Human Cyclase-Associated Proteins 1 and 2 to Promote Actin Severing by Cofilins.International journal of molecular sciences · 2019Article
- Atomic view into Plasmodium actin polymerization, ATP hydrolysis, and fragmentation.PLoS biology · 2019Article
- Mechanism of actin polymerization revealed by cryo-EM structures of actin filaments with three different bound nucleotides.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- ACTIN-DIRECTED TOXIN. ACD toxin-produced actin oligomers poison formin-controlled actin polymerization.Science (New York, N.Y.) · 2015Article
- Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments.Nature structural & molecular biology · 2014Article
- ATP and ADP actin states.Biopolymers · 2013Article
- Rapid nucleotide exchange renders Asp-11 mutant actins resistant to depolymerizing activity of cofilin, leading to dominant toxicity in vivo.The Journal of biological chemistry · 2013Article
- Functional characterization of the human α-cardiac actin mutations Y166C and M305L involved in hypertrophic cardiomyopathy.Cellular and molecular life sciences : CMLS · 2012Article
- Controlling the cortical actin motor.Protoplasma · 2012Review
- The W-loop of alpha-cardiac actin is critical for heart function and endocardial cushion morphogenesis in zebrafish.Molecular and cellular biology · 2012Article
- Structural basis for profilin-mediated actin nucleotide exchange.Journal of molecular biology · 2012Article
- Subdomain location of mutations in cardiac actin correlate with type of functional change.PloS one · 2012Article
- Mutant profilin suppresses mutant actin-dependent mitochondrial phenotype in Saccharomyces cerevisiae.The Journal of biological chemistry · 2011Article
- Myosin binding surface on actin probed by hydroxyl radical footprinting and site-directed labels.Journal of molecular biology · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The nucleotide state of actin (ATP, ADP-P(i), or ADP) is known to impact its interactions with other actin molecules upon polymerization as well as with multiple actin binding proteins both in the monomeric and filamentous states of actin. Recently, molecular dynamics simulations predicted that a sequence located at the interface of subdomains 1 and 3 (W-loop; residues 165-172) changes from an unstructured loop to a beta-turn conformation upon ATP hydrolysis (Zheng, X., Diraviyam, K., and Sept, D. (2007) Biophys. J. 93, 1277-1283). This region participates directly in the binding to other subunits in F-actin as well as to cofilin, profilin, and WH2 domain proteins and, therefore, could contribute to the nucleotide sensitivity of these interactions. The present study demonstrates a reciprocal communication between the W-loop region and the nucleotide binding cleft on actin. Point mutagenesis of residues 167, 169, and 170 and their site-specific labeling significantly affect the nucleotide release from the cleft region, whereas the ATP/ADP switch alters the fluorescence of probes located in the W-loop. In the ADP-P(i) state, the W-loop adopts a conformation similar to that in the ATP state but different from the ADP state. Binding of latrunculin A to the nucleotide cleft favors the ATP-like conformation of the W-loop, whereas ADP-ribosylation of Arg-177 forces the W-loop into a conformation distinct from those in the ADP and ATP-states. Overall, our experimental data suggest that the W-loop of actin is a nucleotide sensor, which may contribute to the nucleotide state-dependent changes in F-actin and nucleotide state-modulated interactions of both G- and F-actin with actin-binding proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.