ArticleInfection and immunity2010
Phagolysosomal integrity is generally maintained after Staphylococcus aureus invasion of nonprofessional phagocytes but is modulated by strain 6850.
Article in Infection and immunity, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Systematic identification of bacterial factors driving Staphylococcus aureus intracellular lifestyle in non-professional phagocytes.Nature communications · 2025Article
- Inhibition of multiple staphylococcal growth states by a small molecule that disrupts membrane fluidity and voltage.mSphere · 2024Article
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- Prevalence of the SigB-Deficient Phenotype among ClinicalAntibiotics (Basel, Switzerland) · 2023Article
- Microscopy-based phenotypic profiling of infection by Staphylococcus aureus clinical isolates reveals intracellular lifestyle as a prevalent feature.Nature communications · 2022Article
- Article
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- Intracellular Habitation ofBiomedicines · 2022Review
- Further Insight into the Mechanism of Human PMN Lysis following Phagocytosis of Staphylococcus aureus.Microbiology spectrum · 2021Article
- Intracellular Staphylococcus aureus employs the cysteine protease staphopain A to induce host cell death in epithelial cells.PLoS pathogens · 2021Article
- An in vitro study on Staphylococcus schweitzeri virulence.Scientific reports · 2021Article
- Autophagy inFrontiers in cellular and infection microbiology · 2021Review
- Time-resolved analysis ofVirulence · 2020Article
- A Convenient Colorimetric Bacteria Detection Method Utilizing Chitosan-Coated Magnetic Nanoparticles.Nanomaterials (Basel, Switzerland) · 2020Article
- Intracellular Environment andFrontiers in microbiology · 2020Article
- Isolating Crucial Steps in Induction of Infective Endocarditis With Preclinical Modeling of Host Pathogen Interaction.Frontiers in microbiology · 2020Article
- The Role of Macrophages inFrontiers in immunology · 2020Review
- Within-host evolution of bovine Staphylococcus aureus selects for a SigB-deficient pathotype characterized by reduced virulence but enhanced proteolytic activity and biofilm formation.Scientific reports · 2019Article
- Survival of Staphylococcus epidermidis in Fibroblasts and Osteoblasts.Infection and immunity · 2018Article
- Intracellular Staphylococcus aureus Modulates Host Central Carbon Metabolism To Activate Autophagy.mSphere · 2018Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococcus aureus is a major cause of a variety of both local and systemic infections. It can invade human host cells, a process that may account for disseminated and recurrent infections. S. aureus postinvasion events in nonprofessional phagocytes are only partially understood. While morphological data suggest a phagosomal escape, there is a lack of corroborating functional data. Using a combination of pH determination and morphological techniques, we have tested the integrity of Staphylococcus-containing phagosomes in 293 (HEK-293), HeLa, and EA.hy926 cells over time. Rapid acidification of S. aureus-containing phagosomes occurred and was sustained for up to 24 h. All S. aureus strains tested displayed equally sustained intraphagosomal pH levels without exhibiting any correlation with pH level and hemolytic activity. The membrane morphology of the phagosomal compartment was heterogeneous, even under conditions where acidic pH was fully maintained, an observation incompatible with phagolysosomal membrane destruction. As an exception, S. aureus strain 6850 showed a reduced phagosomal acidification signal 6 h after invasion. Additionally, only strain 6850 failed to localize to LAMP-1-positive vesicles in HeLa cells, although this was observed only rarely. Several other strongly beta-hemolytic strains did not modulate phagolysosomal pH, suggesting that S. aureus alpha-toxin and beta-toxin are not sufficient for this process. Taken together, our data suggest that S. aureus-containing phagolysosomes generally remain functionally intact in nonprofessional phagocytes, thereby contrasting with transmission electron micrographic results.
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