Evidence map›Paper›PMID 20529880›Full record

ArticleNucleic acids research2010

chipD: a web tool to design oligonucleotide probes for high-density tiling arrays.

Yann S Dufour, Gary E Wesenberg, Andrew J Tritt, Jeremy D Glasner, Nicole T Perna, Julie C Mitchell, Timothy J Donohue

Abstract read
In one paragraph

Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Dual RNA-seq in filarial nematodes andFrontiers in microbiology · 2024
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  11. Comparative genomics of xylose-fermenting fungi for enhanced biofuel production.Proceedings of the National Academy of Sciences of the United States of America · 2011
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yann S DufourDepartment of Bacteriology, University of Wisconsin, Madison, WI 53706, USA. ydufour@wisc.edu
Gary E Wesenberg
Andrew J Tritt
Jeremy D Glasner
Nicole T Perna
Julie C Mitchell
Timothy J Donohue

Funding

Molecular Evolution of Microbial Pathogen GenomesR01GM062994 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI PERNA, NICOLE T · 2001 to 2009
$1.9M
Bacterial Response to Singlet OxygenR01GM075273 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI DONOHUE, TIMOTHY J · 2006 to 2009
$1.1M
NIGMS NIH HHS GM075273NIGMS NIH HHS R01 GM075273NIGMS NIH HHS R01-GM62994
6 · The paper itself

Abstract

chipD is a web server that facilitates design of DNA oligonucleotide probes for high-density tiling arrays, which can be used in a number of genomic applications such as ChIP-chip or gene-expression profiling. The server implements a probe selection algorithm that takes as an input, in addition to the target sequences, a set of parameters that allow probe design to be tailored to specific applications, protocols or the array manufacturer's requirements. The algorithm optimizes probes to meet three objectives: (i) probes should be specific; (ii) probes should have similar thermodynamic properties; and (iii) the target sequence coverage should be homogeneous and avoid significant gaps. The output provides in a text format, the list of probe sequences with their genomic locations, targeted strands and hybridization characteristics. chipD has been used successfully to design tiling arrays for bacteria and yeast. chipD is available at http://chipd.uwbacter.org/.

Indexed as

Oligonucleotide Array Sequence AnalysisSoftwareAlgorithmsGene Expression ProfilingInternetOligonucleotide ProbesRhodobacter sphaeroidesUser-Computer InterfaceOligonucleotide Probes

Identifiers

PMID20529880
PMCPMC2896189

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.