ArticleThe Journal of biological chemistry2010
Vascular endothelial growth factor receptor-2 activates ADP-ribosylation factor 1 to promote endothelial nitric-oxide synthase activation and nitric oxide release from endothelial cells.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 26 citations in OpenAlex.
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- The Microenvironment of Decellularized Extracellular Matrix from Heart Failure Myocardium Alters the Balance between Angiogenic and Fibrotic Signals from Stromal Primitive Cells.International journal of molecular sciences · 2020Article
- Deletion of Robo4 prevents high-fat diet-induced adipose artery and systemic metabolic dysfunction.Microcirculation (New York, N.Y. : 1994) · 2019Article
- Fat-Specific Protein 27 Regulation of Vascular Function in Human Obesity.Journal of the American Heart Association · 2019Article
- Vascular Endothelial Growth Factor-A Exerts Diverse Cellular Effects via Small G Proteins, Rho and Rap.International journal of molecular sciences · 2018Review
- Pro-Angiogenic Effects of Low Dose Ethoxidine in a Murine Model of Ischemic Hindlimb: Correlation between Ethoxidine Levels and Increased Activation of the Nitric Oxide Pathway.Molecules (Basel, Switzerland) · 2017Article
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- The mechanism and function of mitogen-activated protein kinase activation by ARF1.Cellular signalling · 2015Article
- IGF-1 drives chromogranin A secretion via activation of Arf1 in human neuroendocrine tumour cells.Journal of cellular and molecular medicine · 2015Article
- Arf1 and Arf6 promote ventral actin structures formed by acute activation of protein kinase C and Src.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Overexpression of hepatitis B x-interacting protein in HepG2 cells enhances tumor-induced angiogenesis.Molecular and cellular biochemistry · 2012Article
- ADP-ribosylation factor as a novel target for corneal neovascularization regression.Molecular vision · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Vascular endothelial growth factor (VEGF) induces angiogenesis and regulates endothelial function via production and release of nitric oxide (NO), an important signaling molecule. The molecular basis leading to NO production involves phosphatidylinositiol-3 kinase (PI3K), Akt, and endothelial nitric-oxide synthase (eNOS) activation. In this study, we have examined whether small GTP-binding proteins of the ADP-ribosylation factor (ARF) family act as molecular switches to regulate signaling cascades activated by VEGF in endothelial cells. Our results show that this growth factor can promote the rapid and transient activation of ARF1. In endothelial cells, this GTPase is present on dynamic plasma membrane ruffles. Inhibition of ARF1 expression, using RNA interference, markedly impaired VEGF-dependent eNOS phosphorylation and NO production by preventing the activation of the PI3K/Akt signaling axis. Furthermore, our data indicate that phosphorylation of Tyr(801), on VEGF receptor 2, is essential for activating Src- and ARF1-dependent signaling events leading to NO release from endothelial cells. Lastly, this mediator is known to regulate a broad variety of endothelial cell functions. Depletion of ARF1 markedly inhibits VEGF-dependent increase of vascular permeability as well as capillary tubule formation, a process important for angiogenesis. Taken together, our data indicate that ARF1 is a novel modulator of VEGF-stimulated NO release and signaling in endothelial cells.
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Registered trials
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