ArticleThe Journal of biological chemistry2010
The Crc global regulator inhibits the Pseudomonas putida pWW0 toluene/xylene assimilation pathway by repressing the translation of regulatory and structural genes.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 69 citations in OpenAlex.
- Measuring Communication in Microbial Biofilms in Response to Antibiotics, Phytochemicals and Stressors.Antioxidants (Basel, Switzerland) · 2026Article
- The ISPpu9 insertion sequence of Pseudomonas putida KT2440 generates various circular intermediates enabling modular transposition.Nucleic acids research · 2025Article
- Impact of fleQ Deficiency on Resource Allocation and Heterologous Gene Expression in Pseudomonas putida Across Various Growth Media.Microbial biotechnology · 2024Article
- Inactivation of Pseudomonas putida KT2440 pyruvate dehydrogenase relieves catabolite repression and improves the usefulness of this strain for degrading aromatic compounds.Microbial biotechnology · 2024Article
- Simultaneous carbon catabolite repression governs sugar and aromatic co-utilization inApplied and environmental microbiology · 2023Article
- Engineering a Pseudomonas taiwanensis 4-coumarate platform for production of para-hydroxy aromatics with high yield and specificity.Metabolic engineering · 2023Article
- The acetoin assimilation pathway of Pseudomonas putida KT2440 is regulated by overlapping global regulatory elements that respond to nutritional cues.Environmental microbiology · 2023Article
- The importance of understanding the regulation of bacterial metabolism.Environmental microbiology · 2023Article
- Biodegradation of p-xylene-a comparison of three psychrophilic Pseudomonas strains through the lens of gene expression.Environmental science and pollution research international · 2022Article
- Preliminary study on the effect of catabolite repression gene knockout on p-nitrophenol degradation in Pseudomonas putida DLL-E4.PloS one · 2022Article
- Expression of the ISPpu9 transposase of Pseudomonas putida KT2440 is regulated by two small RNAs and the secondary structure of the mRNA 5'-untranslated region.Nucleic acids research · 2021Article
- Unraveling the role of the CbrA histidine kinase in the signal transduction of the CbrAB two-component system in Pseudomonas putida.Scientific reports · 2019Article
- Biodegradation of Volatile Organic Compounds and Their Effects on Biodegradability under Co-Existing Conditions.Microbes and environments · 2017Review
- Transcription factor levels enable metabolic diversification of single cells of environmental bacteria.The ISME journal · 2016Article
- AccR is a master regulator involved in carbon catabolite repression of the anaerobic catabolism of aromatic compounds in Azoarcus sp. CIB.The Journal of biological chemistry · 2014Article
- The global regulator Crc plays a multifaceted role in modulation of type III secretion system in Pseudomonas aeruginosa.MicrobiologyOpen · 2013Article
- Catabolite repression control of pyocyanin biosynthesis at an intersection of primary and secondary metabolism in Pseudomonas aeruginosa.Applied and environmental microbiology · 2012Article
- Involvement of the global Crp regulator in cyclic AMP-dependent utilization of aromatic amino acids by Pseudomonas putida.Journal of bacteriology · 2012Article
- The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene.BMC systems biology · 2011Article
- Promoter recognition and activation by the global response regulator CbrB in Pseudomonas aeruginosa.Journal of bacteriology · 2011Article
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Authors and funding
3 authors at 2 institutions in 1 country.
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Abstract
In Pseudomonas putida, the expression of the pWW0 plasmid genes for the toluene/xylene assimilation pathway (the TOL pathway) is subject to complex regulation in response to environmental and physiological signals. This includes strong inhibition via catabolite repression, elicited by the carbon sources that the cells prefer to hydrocarbons. The Crc protein, a global regulator that controls carbon flow in pseudomonads, has an important role in this inhibition. Crc is a translational repressor that regulates the TOL genes, but how it does this has remained unknown. This study reports that Crc binds to sites located at the translation initiation regions of the mRNAs coding for XylR and XylS, two specific transcription activators of the TOL genes. Unexpectedly, eight additional Crc binding sites were found overlapping the translation initiation sites of genes coding for several enzymes of the pathway, all encoded within two polycistronic mRNAs. Evidence is provided supporting the idea that these sites are functional. This implies that Crc can differentially modulate the expression of particular genes within polycistronic mRNAs. It is proposed that Crc controls TOL genes in two ways. First, Crc inhibits the translation of the XylR and XylS regulators, thereby reducing the transcription of all TOL pathway genes. Second, Crc inhibits the translation of specific structural genes of the pathway, acting mainly on proteins involved in the first steps of toluene assimilation. This ensures a rapid inhibitory response that reduces the expression of the toluene/xylene degradation proteins when preferred carbon sources become available.
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