ArticleThe Journal of biological chemistry2010
Zonadhesin is essential for species specificity of sperm adhesion to the egg zona pellucida.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 89 citations in OpenAlex.
- Beyond Shedding: Conserved Retention of ZAN After the Acrosome Reaction Across Rabbit, Boar, and Bull Sperm.International journal of molecular sciences · 2026Article
- TEX29 is a novel acrosome marker dispensable for spermatogenesis and fertilization in mice.Journal of assisted reproduction and genetics · 2026Article
- Molecular Modeling and Gene Ontology Implicate SLC35F4 and SLC35F5 as Golgi-Associated Importers of Flavin-Adenine-Dinucleotide.International journal of molecular sciences · 2026Article
- Functional Methods for Studying Sperm-Zona Pellucida Interaction in Mammals.Methods and protocols · 2025Review
- Proteomic Alterations and Oxidative Stress in Seminal Plasma of Nellore Bulls Under Sexual Rest.International journal of molecular sciences · 2025Article
- Increased reproductive outcomes after optimized sperm preparation.Frontiers in cell and developmental biology · 2025Article
- Article
- SMA20/PMIS2 Is a Rapidly Evolving Sperm Membrane Alloantigen with Possible Species-Divergent Function in Fertilization.International journal of molecular sciences · 2024Article
- Reproductive genomics of the mouse: implications for human fertility and infertility.Development (Cambridge, England) · 2023Article
- Testis-Specific Isoform of NaInternational journal of molecular sciences · 2022Review
- Rapid divergence of a gamete recognition gene promoted macroevolution of Eutheria.Genome biology · 2022Article
- Oviducal gland transcriptomics ofPeerJ · 2022Article
- Prioritising positively selected variants in whole-genome sequencing data using FineMAV.BMC bioinformatics · 2021Article
- Review
- ACRBP (Sp32) is involved in priming sperm for the acrosome reaction and the binding of sperm to the zona pellucida in a porcine model.PloS one · 2021Article
- Protamine and other proteins in sperm and seminal plasma as molecular markers of bull fertility.Veterinary world · 2020Review
- Article
- The Ly6/uPAR protein Bouncer is necessary and sufficient for species-specific fertilization.Science (New York, N.Y.) · 2018Article
- Gamete compatibility genes in mammals: candidates, applications and a potential path forward.Royal Society open science · 2017Review
- Functional Amyloids in Reproduction.Biomolecules · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 5 institutions in 2 countries.
Funding
Abstract
Interaction of rapidly evolving molecules imparts species specificity to sperm-egg recognition in marine invertebrates, but it is unclear whether comparable interactions occur during fertilization in any vertebrate species. In mammals, the sperm acrosomal protein zonadhesin is a rapidly evolving molecule with species-specific binding activity for the egg zona pellucida (ZP). Here we show using null mice produced by targeted disruption of Zan that zonadhesin confers species specificity to sperm-ZP adhesion. Sperm capacitation selectively exposed a partial von Willebrand D domain of mouse zonadhesin on the surface of living, motile cells. Antibodies to the exposed domain inhibited adhesion of wild-type spermatozoa to the mouse ZP but did not inhibit adhesion of spermatozoa lacking zonadhesin. Zan(-/-) males were fertile, and their spermatozoa readily fertilized mouse eggs in vitro. Remarkably, however, loss of zonadhesin increased adhesion of mouse spermatozoa to pig, cow, and rabbit ZP but not mouse ZP. We conclude that zonadhesin mediates species-specific ZP adhesion, and Zan(-/-) males are fertile because their spermatozoa retain adhesion capability that is not species-specific. Mammalian sperm-ZP adhesion is therefore molecularly robust, and species-specific egg recognition by a protein in the sperm acrosome is conserved between invertebrates and vertebrates, even though the adhesion molecules themselves are unrelated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.