ArticleThe Journal of biological chemistry2010
Binding of cysteine synthase to the STAS domain of sulfate transporter and its regulatory consequences.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Sulfur in dialogue with phosphorus, nitrogen, and iron: regulatory networks in plant nutrient homeostasis.Journal of experimental botany · 2026Review
- Comprehensive Characterization ofGenes · 2026Article
- Molecular mechanism underlying phosphate distribution by SULTR family transporter SPDT inScience advances · 2025Article
- Inorganic sulfate is critical forProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genome-Wide Identification of the Sulfate Transporter Gene Family Reveals ThatAntioxidants (Basel, Switzerland) · 2025Article
- Molecular insights into the elevator-type mechanism of the cyanobacterial bicarbonate transporter BicA.Biophysical journal · 2025Article
- SLC26 Anion Transporters.Handbook of experimental pharmacology · 2024Review
- Genome-Wide Characterization of the Sulfate Transporter Gene Family in Oilseed Crops:Plants (Basel, Switzerland) · 2023Article
- Article
- Sulfur signaling and starvation response in Arabidopsis.iScience · 2022Review
- Role of Sulfate Transporters in Chromium Tolerance inPlants (Basel, Switzerland) · 2022Article
- Micrografting Provides Evidence for Systemic Regulation of Sulfur Metabolism between Shoot and Root.Plants (Basel, Switzerland) · 2021Article
- Hydrogen sulfide (HaBIOTECH · 2021Review
- Genome-Wide Identification of Sultr Genes inFrontiers in plant science · 2021Article
- Cysteine and Methionine Biosynthetic Enzymes Have Distinct Effects on Seed Nutritional Quality and on Molecular Phenotypes Associated With Accumulation of a Methionine-Rich Seed Storage Protein in Rice.Frontiers in plant science · 2020Article
- Plant Sulfate Transporters in the Low Phytic Acid Network: Some Educated Guesses.Plants (Basel, Switzerland) · 2019Article
- Structural mechanism of the active bicarbonate transporter from cyanobacteria.Nature plants · 2019Article
- Transcriptome-wide comparison of selenium hyperaccumulator and nonaccumulator Stanleya species provides new insight into key processes mediating the hyperaccumulation syndrome.Plant biotechnology journal · 2018Article
- The E. coli dicarboxylic acid transporters DauA act as a signal transducer by interacting with the DctA uptake system.Scientific reports · 2017Article
- Genetic, metabolomic and transcriptomic analyses of the de novo L-cysteine biosynthetic pathway in the enteric protozoan parasite Entamoeba histolytica.Scientific reports · 2017Article
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Abstract
The sulfate ion (SO(4)(2-)) is transported into plant root cells by SO(4)(2-) transporters and then mostly reduced to sulfide (S(2-)). The S(2-) is then bonded to O-acetylserine through the activity of cysteine synthase (O-acetylserine (thiol)lyase or OASTL) to form cysteine, the first organic molecule of the SO(4)(2-) assimilation pathway. Here, we show that a root plasma membrane SO(4)(2-) transporter of Arabidopsis, SULTR1;2, physically interacts with OASTL. The interaction was initially demonstrated using a yeast two-hybrid system and corroborated by both in vivo and in vitro binding assays. The domain of SULTR1;2 shown to be important for association with OASTL is called the STAS domain. This domain is at the C terminus of the transporter and extends from the plasma membrane into the cytoplasm. The functional relevance of the OASTL-STAS interaction was investigated using yeast mutant cells devoid of endogenous SO(4)(2-) uptake activity but co-expressing SULTR1;2 and OASTL. The analysis of SO(4)(2-) transport in these cells suggests that the binding of OASTL to the STAS domain in this heterologous system negatively impacts transporter activity. In contrast, the activity of purified OASTL measured in vitro was enhanced by co-incubation with the STAS domain of SULTR1;2 but not with the analogous domain of the SO(4)(2-) transporter isoform SULTR1;1, even though the SULTR1;1 STAS peptide also interacts with OASTL based on the yeast two-hybrid system and in vitro binding assays. These observations suggest a regulatory model in which interactions between SULTR1;2 and OASTL coordinate internalization of SO(4)(2-) with the energetic/metabolic state of plant root cells.
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