ArticleThe Journal of biological chemistry2010
Two Golgi-resident 3'-Phosphoadenosine 5'-phosphosulfate transporters play distinct roles in heparan sulfate modifications and embryonic and larval development in Caenorhabditis elegans.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- Cylindracin, a Fruiting Body-Specific Protein ofToxins · 2025Article
- Ketamine induces apical extracellular matrix modifications in Caenorhabditis elegans.Scientific reports · 2022Article
- Chondroitin 4-O-Sulfotransferase Is Indispensable for Sulfation of Chondroitin and Plays an Important Role in Maintaining Normal Life Span and Oxidative Stress Responses in Nematodes.The Journal of biological chemistry · 2016Article
- Inhibition of Lithium-Sensitive Phosphatase BPNT-1 Causes Selective Neuronal Dysfunction in C. elegans.Current biology : CB · 2016Article
- Article
- RNAi screening of human glycogene orthologs in the nematode Caenorhabditis elegans and the construction of the C. elegans glycogene database.Glycobiology · 2015Article
- An organelle gatekeeper function for Caenorhabditis elegans UNC-16 (JIP3) at the axon initial segment.Genetics · 2013Article
- GPI-anchor synthesis is indispensable for the germline development of the nematode Caenorhabditis elegans.Molecular biology of the cell · 2012Article
- Genetic analysis of the heparan modification network in Caenorhabditis elegans.The Journal of biological chemistry · 2011Article
- The Caenorhabditis elegans epidermis as a model skin. II: differentiation and physiological roles.Wiley interdisciplinary reviews. Developmental biologyReview
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Authors and funding
12 authors at 5 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Synthesis of extracellular sulfated molecules requires active 3'-phosphoadenosine 5'-phosphosulfate (PAPS). For sulfation to occur, PAPS must pass through the Golgi membrane, which is facilitated by Golgi-resident PAPS transporters. Caenorhabditis elegans PAPS transporters are encoded by two genes, pst-1 and pst-2. Using the yeast heterologous expression system, we characterized PST-1 and PST-2 as PAPS transporters. We created deletion mutants to study the importance of PAPS transporter activity. The pst-1 deletion mutant exhibited defects in cuticle formation, post-embryonic seam cell development, vulval morphogenesis, cell migration, and embryogenesis. The pst-2 mutant exhibited a wild-type phenotype. The defects observed in the pst-1 mutant could be rescued by transgenic expression of pst-1 and hPAPST1 but not pst-2 or hPAPST2. Moreover, the phenotype of a pst-1;pst-2 double mutant were similar to those of the pst-1 single mutant, except that larval cuticle formation was more severely defected. Disaccharide analysis revealed that heparan sulfate from these mutants was undersulfated. Gene expression reporter analysis revealed that these PAPS transporters exhibited different tissue distributions and subcellular localizations. These data suggest that pst-1 and pst-2 play different physiological roles in heparan sulfate modification and development.
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