ArticleAnalytical chemistry2010
Fabrication of uniform DNA-conjugated hydrogel microparticles via replica molding for facile nucleic acid hybridization assays.
Article in Analytical chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed.
- Stimuli-Responsive Hydrogels: From Swelling-Deswelling Mechanisms to Biomedical Applications.Nanomaterials (Basel, Switzerland) · 2026Review
- Air-through-precursor suction-augmented replica molding for fabrication of anisotropic microparticles in gas-impermeable molds.RSC advances · 2024Article
- Lab on a Particle Technologies.Analytical chemistry · 2024Review
- Facile Microfluidic Fabrication of Biocompatible Hydrogel Microspheres in a Novel Microfluidic Device.Molecules (Basel, Switzerland) · 2022Article
- Recent Advances in Polymer Additive Engineering for Diagnostic and Therapeutic Hydrogels.International journal of molecular sciences · 2022Review
- Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors.ACS sensors · 2021Review
- A platform for multiplexed colorimetric microRNA detection using shape-encoded hydrogel particles.The Analyst · 2020Article
- Engineering functional hydrogel microparticle interfaces by controlled oxygen-inhibited photopolymerization.Colloids and surfaces. B, Biointerfaces · 2019Article
- Bioengineering Microgels and Hydrogel Microparticles for Sensing Biomolecular Targets.Gels (Basel, Switzerland) · 2017Review
- Hydrogel microparticles for biosensing.European polymer journal · 2015Article
- High-Throughput Contact Flow Lithography.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2015Article
- Oil-isolated hydrogel microstructures for sensitive bioassays on-chip.Analytical chemistry · 2013Article
- Responsive DNA-based hydrogels and their applications.Macromolecular rapid communications · 2013Review
- Reductively responsive siRNA-conjugated hydrogel nanoparticles for gene silencing.Journal of the American Chemical Society · 2012Article
- Quantitative experimental determination of primer-dimer formation risk by free-solution conjugate electrophoresis.Electrophoresis · 2012Article
- Multiplex immunoassay platforms based on shape-coded poly(ethylene glycol) hydrogel microparticles incorporating acrylic acid.Sensors (Basel, Switzerland) · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We identify and investigate several critical parameters in the fabrication of single-stranded DNA conjugated poly(ethylene glycol) (PEG) microparticles based on replica molding (RM) for highly uniform and robust nucleic acid hybridization assays. The effects of PEG-diacrylate, probe DNA, and photoinitiator concentrations on the overall fluorescence and target DNA penetration depth upon hybridization are examined. Fluorescence and confocal microscopy results illustrate high conjugation capacity of the probe and target DNA, femtomole sensitivity, and sequence specificity. Combined, these findings demonstrate a significant step toward simple, robust, and scalable procedures to manufacture highly uniform and high-capacity hybridization assay particles in a well-controlled manner by exploiting many advantages that the batch processing-based RM technique offers. We envision that the results presented here may be readily applied to rapid and high-throughput hybridization assays for a wide variety of applications in bioprocess monitoring, food safety, and biological threat detection.
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Registered trials
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