ArticleCellular and molecular bioengineering2010
The Effects of Load on E-Selectin Bond Rupture and Bond Formation.
Article in Cellular and molecular bioengineering, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Trial
- Dual Affinity Nanoparticles for the Transport of Therapeutics from Carrier Cells to Target Cells under Physiological Flow Conditions.ACS omega · 2023Article
- Molecular mechanisms of catch bonds and their implications for platelet hemostasis.Biophysical reviews · 2023Review
- What's the Catch? The Significance of Catch Bonds in T Cell Activation.Journal of immunology (Baltimore, Md. : 1950) · 2023Review
- Receptor-Ligand Binding: Effect of Mechanical Factors.International journal of molecular sciences · 2023Review
- Recombinant FimH Adhesin Demonstrates How the Allosteric Catch Bond Mechanism Can Support Fast and Strong Bacterial Attachment in the Absence of Shear.Journal of molecular biology · 2022Article
- Quantitative interpretation of cell rolling velocity distribution.Biophysical journal · 2021Article
- Targeting Neutrophil Adhesive Events to Address Vaso-Occlusive Crisis in Sickle Cell Patients.Frontiers in immunology · 2021Review
- Mapping cell surface adhesion by rotation tracking and adhesion footprinting.Scientific reports · 2017Article
- A Rough Energy Landscape to Describe Surface-Linked Antibody and Antigen Bond Formation.Scientific reports · 2016Article
- E-selectin ligand complexes adopt an extended high-affinity conformation.Journal of molecular cell biology · 2016Article
- Loop 2 of myosin is a force-dependent inhibitor of the rigor bond.Journal of muscle research and cell motility · 2014Article
- Targeting Underglycosylated MUC1 for the Selective Capture of Highly Metastatic Breast Cancer Cells Under Flow.Cellular and molecular bioengineering · 2013Article
- A High-Throughput Technique Reveals the Load- and Site Density-Dependent Kinetics of E-Selectin.Cellular and molecular bioengineering · 2012Article
- Kinetics and mechanics of two-dimensional interactions between T cell receptors and different activating ligands.Biophysical journal · 2012Article
- The two-pathway model of the biological catch-bond as a limit of the allosteric model.Biophysical journal · 2011Article
- Force spectroscopy reveals multiple "closed states" of the muscle thin filament.The Journal of biological chemistry · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Molecular dissociation rates have long been known to be sensitive to applied force. We use a laser trap to provide evidence that rates of association may also be force-dependent. We use the thermal fluctuation assay to study single bonds between E-selectin and sialyl Lewis(a) (sLe(a)), the sugar on PSGL-1 to which the three selectins bind. Briefly, an E-selectin-coated bead is held in a laser trap and pressed with various compressive loads against the vertical surface of a bead coated with sLe(a). The time it takes for a bond to form is used to calculate a specific two-dimensional on-rate, kono. We observe an increase in kono with increasing compressive force, providing single molecule evidence that on-rate, in addition to off-rate, is influenced by load. By measuring bond lifetimes at known tensile loads, we show that E-selectin, like its family members L- and P-selectin, is capable of forming catch bonds. Our data support a reverse Bell model, in which compressive forces lower the activation energy for binding. Load-dependent on-rates may be a general feature of all intermolecular bonds.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.