ArticlePloS one2010
Industrial melanism in the peppered moth is not associated with genetic variation in canonical melanisation gene candidates.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Tyrosine Hydroxylase and DOPA Decarboxylase Are Associated With Pupal Melanization During Larval-Pupal Transformation inFrontiers in physiology · 2022Article
- Comparative transcriptomics of albino and warningly-coloured caterpillars.Ecology and evolution · 2021Article
- A New Diagnostic Resource forG3 (Bethesda, Md.) · 2017Article
- A Chromosome-Scale Assembly of theG3 (Bethesda, Md.) · 2017Article
- Article
- Evolutionary biosemiotics and multilevel construction networks.Biosemiotics · 2016Article
- Article
- Overexpression of Tyrosine hydroxylase and Dopa decarboxylase associated with pupal melanization in Spodoptera exigua.Scientific reports · 2015Article
- Aspartate Decarboxylase is Required for a Normal Pupa Pigmentation Pattern in the Silkworm, Bombyx mori.Scientific reports · 2015Article
- Pupal melanization is associated with higher fitness in Spodoptera exigua.Scientific reports · 2015Article
- Evolutionary constraints or opportunities?Bio Systems · 2014Article
- Phenylalanine metabolism regulates reproduction and parasite melanization in the malaria mosquito.PloS one · 2014Article
- The peppered moth and industrial melanism: evolution of a natural selection case study.Heredity · 2013Review
- Linkage map of the peppered moth, Biston betularia (Lepidoptera, Geometridae): a model of industrial melanism.Heredity · 2013Article
- Genetic basis of stage-specific melanism: a putative role for a cysteine sulfinic acid decarboxylase in insect pigmentation.Heredity · 2012Article
- Genetic linkage between melanism and winglessness in the ladybird beetle Adalia bipunctata.Genetica · 2012Article
- Convergent, modular expression of ebony and tan in the mimetic wing patterns of Heliconius butterflies.Development genes and evolution · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Industrial melanism in the peppered moth (Biston betularia) is an iconic case study of ecological genetics but the molecular identity of the gene determining the difference between the typical and melanic (carbonaria) morphs is entirely unknown. We applied the candidate gene approach to look for associations between genetic polymorphisms within sixteen a priori melanisation gene candidates and the carbonaria morph. The genes were isolated and sequence characterised in B. betularia using degenerate PCR and from whole-transcriptome sequence. The list of candidates contains all the genes previously implicated in melanisation pattern differences in other insects, including aaNAT, DOPA-decarboxylase, ebony, tan, tyrosine hydroxylase, yellow and yellow2 (yellow-fa). Co-segregation of candidate gene alleles and carbonaria morph was tested in 73 offspring of a carbonaria male-typical female backcross. Surprisingly, none of the sixteen candidate genes was in close linkage with the locus controlling the carbonaria-typical polymorphism. Our study demonstrates that the 'carbonaria gene' is not a structural variant of a canonical melanisation pathway gene, neither is it a cis-regulatory element of these enzyme-coding genes. The implication is either that we have failed to characterize an unknown enzyme-coding gene in the melanisation pathway, or more likely, that the 'carbonaria gene' is a higher level trans-acting factor which regulates the spatial expression of one or more of the melanisation candidates in this study to alter the pattern of melanin production.
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