ArticleMetabolomics : Official journal of the Metabolomic Society2010
H NMR-based metabolomics combined with HPLC-PDA-MS-SPE-NMR for investigation of standardized Ginkgo biloba preparations.
Article in Metabolomics : Official journal of the Metabolomic Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 80 citations in OpenAlex.
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- Pharmacokinetic, Metabolism, and Metabolomic Strategies Provide Deep Insight Into the Underlying Mechanism ofFrontiers in nutrition · 2022Review
- You Are What You Eat: Application of Metabolomics Approaches to Advance Nutrition Research.Foods (Basel, Switzerland) · 2021Review
- PSYCHE-A Valuable Experiment in Plant NMR-Metabolomics.Molecules (Basel, Switzerland) · 2020Article
- A nontargeted approach to determine the authenticity of Ginkgo biloba L. plant materials and dried leaf extracts by liquid chromatography-high-resolution mass spectrometry (LC-HRMS) and chemometrics.Analytical and bioanalytical chemistry · 2020Article
- Metabolomics in the Context of Plant Natural Products Research: From Sample Preparation to Metabolite Analysis.Metabolites · 2020Review
- Integrated analytical assets aid botanical authenticity and adulteration management.Fitoterapia · 2018Review
- Quantification of terpene trilactones in Ginkgo biloba with aJournal of natural medicines · 2018Article
- Facilitated Visual Interpretation of Scores in Principal Component Analysis by Bioactivity-Labeling of 1H-NMR Spectra-Metabolomics Investigation and Identification of a New α-Glucosidase Inhibitor in Radix Astragali.Molecules (Basel, Switzerland) · 2017Article
- Two dimensional NMR spectroscopic approaches for exploring plant metabolome: A review.Journal of advanced research · 2015Review
- The tandem of full spin analysis and qHNMR for the quality control of botanicals exemplified with Ginkgo biloba.Journal of natural products · 2012Article
- Systems biology of meridians, acupoints, and chinese herbs in disease.Evidence-based complementary and alternative medicine : eCAM · 2012Article
- NMR Metabolomics Analysis of Parkinson's Disease.Current MetabolomicsArticle
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Commercial preparations of Ginkgo biloba are very complex mixtures prepared from raw leaf extracts by a series of extraction and prepurification steps. The pharmacological activity is attributed to a number of flavonoid glycosides and unique terpene trilactones (TTLs), with largely uncharacterized pharmacological profiles on targets involved in neurological disorders. It is therefore important to complement existing targeted analytical methods for analysis of Ginkgo biloba preparations with alternative technology platforms for their comprehensive and global characterization. In this work, (1)H NMR-based metabolomics and hyphenation of high-performance liquid chromatography, photo-diode array detection, mass spectrometry, solid-phase extraction, and nuclear magnetic resonance spectroscopy (HPLC-PDA-MS-SPE-NMR) were used for investigation of 16 commercially available preparations of Ginkgo biloba. The standardized extracts originated from Denmark, Italy, Sweden, and United Kingdom, and the results show that (1)H NMR spectra allow simultaneous assessment of the content as well as identity of flavonoid glycosides and TTLs based on a very simple sample-preparation procedure consisting of extraction, evaporation and reconstitution in acetone-d(6). Unexpected or unwanted extract constituents were also easily identified in the (1)H NMR spectra, which contrasts traditional methods that depend on UV absorption or MS ionizability and usually require availability of reference standards. Automated integration of (1)H NMR spectral segments (buckets or bins of 0.02 ppm width) provides relative distribution plots of TTLs based on their H-12 resonances. The present study shows that (1)H NMR-based metabolomics is an attractive method for non-selective and comprehensive analysis of Ginkgo extracts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-009-0195-x) contains supplementary material, which is available to authorized users.
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Registered trials
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