ArticleMolecular & cellular proteomics : MCP2010
The SILAC fly allows for accurate protein quantification in vivo.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 172 citations in OpenAlex.
- Evolution of the reproductive interactome and the origins of postmating-prezygotic reproductive divergence.BMC biology · 2026Article
- Differential regulation of the proteome and phosphoproteome along the dorso-ventral axis of the earlyeLife · 2024Article
- A Tutorial Review of Labeling Methods in Mass Spectrometry-Based Quantitative Proteomics.ACS measurement science au · 2024Review
- Females translate male mRNA transferred during mating.iScience · 2024Article
- Cardiolipin prolongs the lifetimes of respiratory proteins in Drosophila flight muscle.The Journal of biological chemistry · 2023Article
- SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The life history ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Using stable isotope labeling to advance our understanding of Alzheimer's disease etiology and pathology.Journal of neurochemistry · 2021Review
- Nucleotide sugar biosynthesis occurs in the glycosomes of procyclic and bloodstream form Trypanosoma brucei.PLoS neglected tropical diseases · 2021Article
- Recurrent Topics in Mass Spectrometry-Based Metabolomics and Lipidomics-Standardization, Coverage, and Throughput.Analytical chemistry · 2021Review
- Quantitative Mass Spectrometry-Based Proteomics: An Overview.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Stable Isotope Labeling of Amino Acids in Flies (SILAF) Reveals Differential Phosphorylation of Mitochondrial Proteins Upon Loss of OXPHOS Subunits.Molecular & cellular proteomics : MCP · 2021Article
- TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis.Journal of visualized experiments : JoVE · 2020Article
- Integrative Metabolic Pathway Analysis Reveals Novel Therapeutic Targets in Osteoarthritis.Molecular & cellular proteomics : MCP · 2020Article
- Proteome turnover in the bloodstream and procyclic forms ofWellcome open research · 2019Article
- Screening differentially expressed proteins from co-cultured hematopoietic cells and bone marrow-derived stromal cells by quantitative proteomics (SILAC) method.Clinical proteomics · 2019Article
- The developmental proteome ofGenome research · 2017Article
- Exploring ribosome composition and newly synthesized proteins through proteomics and potential biomedical applications.Expert review of proteomics · 2017Review
- Matching Dietary Amino Acid Balance to the In Silico-Translated Exome Optimizes Growth and Reproduction without Cost to Lifespan.Cell metabolism · 2017Article
- A Biologist's Field Guide to Multiplexed Quantitative Proteomics.Molecular & cellular proteomics : MCP · 2016Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stable isotope labeling by amino acids in cell culture (SILAC) is widely used to quantify protein abundance in tissue culture cells. Until now, the only multicellular organism completely labeled at the amino acid level was the laboratory mouse. The fruit fly Drosophila melanogaster is one of the most widely used small animal models in biology. Here, we show that feeding flies with SILAC-labeled yeast leads to almost complete labeling in the first filial generation. We used these "SILAC flies" to investigate sexual dimorphism of protein abundance in D. melanogaster. Quantitative proteome comparison of adult male and female flies revealed distinct biological processes specific for each sex. Using a tudor mutant that is defective for germ cell generation allowed us to differentiate between sex-specific protein expression in the germ line and somatic tissue. We identified many proteins with known sex-specific expression bias. In addition, several new proteins with a potential role in sexual dimorphism were identified. Collectively, our data show that the SILAC fly can be used to accurately quantify protein abundance in vivo. The approach is simple, fast, and cost-effective, making SILAC flies an attractive model system for the emerging field of in vivo quantitative proteomics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.