ArticleProceedings of the National Academy of Sciences of the United States of America1991
Parallel pathways of cell cycle control during Xenopus egg activation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 33 citations in OpenAlex.
- Protein kinase C isoforms α, δ and ε are differentially expressed in mouse ovaries at different stages of postnatal development.Journal of ovarian research · 2014Article
- Zinc depletion activates porcine metaphase II oocytes independently of the protein kinase C pathway.In vitro cellular & developmental biology. Animal · 2014Article
- Actin-dependent propulsion of endosomes and lysosomes by recruitment of N-WASP.The Journal of cell biology · 2000Article
- sn-1,2-diacylglycerol and choline increase after fertilization in Xenopus laevis.Molecular biology of the cell · 1997Article
- Cell division arrest induced by phorbol ester in CHO cells overexpressing protein kinase C-delta subspecies.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Transit from M phase into interphase in many eukaryotic cells is preceded by an increase in intracellular free calcium ([Ca2+]i), which may act via calcium-dependent enzymes to trigger the M-phase/interphase transition. To test the role of the calcium- and phospholipid-dependent enzyme protein kinase C (PKC) in the M-phase/interphase transition, PKC was activated in M-phase-arrested Xenopus eggs by treatment with the phorbol ester phorbol 12-myristate 13-acetate under conditions that prevent a rise in [Ca2+]i and activation of other calcium-dependent enzymes. Under these conditions, several cellular events characteristic of transit into interphase occur: sperm chromatin decondenses, the Golgi and the nuclear envelope reassemble, and endocytosis resumes. These events are also triggered by treatment of eggs with the diacylglycerol 1,2-dioctanoyl-sn-glycerol. Surprisingly, the activity of M-phase-promoting factor (MPF), a universal regulator of M phase, remains high under these conditions. If [Ca2+]i is subsequently raised, MPF activity is rapidly destroyed. Similarly, lysates made from eggs treated with phorbol 12-myristate 13-acetate support sperm chromatin decondensation in vitro and yet retain high MPF activity, measured either as the ability to induce meiotic resumption in oocytes or as histone H1 kinase activity. These effects are not triggered by the 4 alpha-phorbol ester isomer, which does not activate PKC, and are sensitive to the PKC "pseudosubstrate" peptide. The results suggest that two, parallel signals are generated by the rise in [Ca2+]i both of which contribute to cell cycle regulation. One pathway inactivates MPF; the other pathway activates PKC.
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