ArticleApplied and environmental microbiology2010
Redesign, reconstruction, and directed extension of the Brevibacterium linens C40 carotenoid pathway in Escherichia coli.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Sporobolomyces pararoseus: a versatile chassis for circular bioproduction of β-carotene, torulene, and torularhodin.Archives of microbiology · 2026Review
- Genomic Insights into the Role of cAMP in Carotenoid Biosynthesis: Enhancing β-Carotene Production inInternational journal of molecular sciences · 2024Article
- Complete microbial synthesis of crocetin and crocins from glycerol in Escherichia coli.Microbial cell factories · 2024Article
- Draft genome sequence of a new carotenoid-producing strain3 Biotech · 2022Article
- Plasma membrane phylloquinone biosynthesis in nonphotosynthetic parasitic plants.Plant physiology · 2021Article
- Genome Mining Reveals Two Missing CrtP and AldH Enzymes in the C30 Carotenoid Biosynthesis Pathway inMolecules (Basel, Switzerland) · 2020Article
- Multiplicity of carotene patterns derives from competition between phytoene desaturase diversification and biological environments.Scientific reports · 2020Article
- Microbial Production of Retinyl Palmitate and Its Application as a Cosmeceutical.Antioxidants (Basel, Switzerland) · 2020Article
- Article
- Redesign and reconstruction of a steviol-biosynthetic pathway for enhanced production of steviol in Escherichia coli.Microbial cell factories · 2020Article
- Engineering and application of syntheticBiotechnology for biofuels · 2018Article
- Complete Genome Sequence of Brevibacterium linens SMQ-1335.Genome announcements · 2016Article
- Generation of structurally novel short carotenoids and study of their biological activity.Scientific reports · 2016Article
- Sioxanthin, a novel glycosylated carotenoid, reveals an unusual subclustered biosynthetic pathway.Environmental microbiology · 2015Article
- Mechanistic aspects of carotenoid biosynthesis.Chemical reviews · 2014Review
- Reconstruction of the carotenoid biosynthetic pathway of Cronobacter sakazakii BAA894 in Escherichia coli.PloS one · 2014Article
- New insight into the cleavage reaction of Nostoc sp. strain PCC 7120 carotenoid cleavage dioxygenase in natural and nonnatural carotenoids.Applied and environmental microbiology · 2013Article
- Heterologous carotenoid-biosynthetic enzymes: functional complementation and effects on carotenoid profiles in Escherichia coli.Applied and environmental microbiology · 2013Article
- Functional expression and extension of staphylococcal staphyloxanthin biosynthetic pathway in Escherichia coli.The Journal of biological chemistry · 2012Article
- Novel activity of Rhodobacter sphaeroides spheroidene monooxygenase CrtA expressed in Escherichia coli.Applied and environmental microbiology · 2010Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, the carotenoid biosynthetic pathways of Brevibacterium linens DSMZ 20426 were reconstructed, redesigned, and extended with additional carotenoid-modifying enzymes of other sources in a heterologous host Escherichia coli. The modular lycopene pathway synthesized an unexpected carotenoid structure, 3,4-didehydrolycopene, as well as lycopene. Extension of the novel 3,4-didehydrolycopene pathway with the mutant Pantoea lycopene cyclase CrtY(2) and the Rhodobacter spheroidene monooxygenase CrtA generated monocyclic torulene and acyclic oxocarotenoids, respectively. The reconstructed beta-carotene pathway synthesized an unexpected 7,8-dihydro-beta-carotene in addition to beta-carotene. Extension of the beta-carotene pathway with the B. linens beta-ring desaturase CrtU and Pantoea beta-carotene hydroxylase CrtZ generated asymmetric carotenoid agelaxanthin A, which had one aromatic ring at the one end of carotene backbone and one hydroxyl group at the other end, as well as aromatic carotenoid isorenieratene and dihydroxy carotenoid zeaxanthin. These results demonstrate that reconstruction of the biosynthetic pathways and extension with promiscuous enzymes in a heterologous host holds promise as a rational strategy for generating structurally diverse compounds that are hardly accessible in nature.
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