ArticleThe Plant cell2010
Phospholipase A(2) is required for PIN-FORMED protein trafficking to the plasma membrane in the Arabidopsis root.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 104 citations in OpenAlex.
- Lysophosphatidylcholine homeostasis via the Lands cycle regulates root growth in Arabidopsis.Plant & cell physiology · 2026Article
- Phospholipid Networks as Metabolic Hubs and Signaling Integrators in Plant Development and Stress Adaptation.Plants (Basel, Switzerland) · 2026Review
- ROPGAP3 interacts with PIN2 and modulates its clustering and trafficking inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- De novo transcriptome analysis and functional annotation of Silybum Marianum L. under drought stress with a focus on Silymarin synthesis and MAPK signaling pathways.BMC plant biology · 2025Article
- The non-specific phospholipase C of common bean PvNPC4 modulates roots and nodule development.PloS one · 2025Article
- Overexpression of the PotatoInternational journal of molecular sciences · 2024Article
- Comparative Metabolome and Transcriptome Analysis Reveals the Possible Roles of Rice Phospholipase A Genes in the Accumulation of Oil in Grains.International journal of molecular sciences · 2024Article
- Lyso-phosphatidylethanolamine triggers immunity against necrotrophs by promoting JA-signaling and ROS-homeostasis.Plant molecular biology · 2023Article
- Comparative phylogenomic and structural analysis of canonical secretory PLA2 and novel PLA2-like family in plants.Frontiers in plant science · 2023Article
- Nitrated Fatty-Acids Distribution in Storage Biomolecules duringAntioxidants (Basel, Switzerland) · 2022Article
- Overexpression ofPlants (Basel, Switzerland) · 2022Article
- The Reduced Longitudinal Growth Induced by Overexpression ofPlants (Basel, Switzerland) · 2021Article
- Calcium-dependent protein kinase 29 modulates PIN-FORMED polarity and Arabidopsis development via its own phosphorylation code.The Plant cell · 2021Article
- Lyso-phosphatidylethanolamine primes the plant immune system and promotes basal resistance against hemibiotrophic pathogens.BMC biotechnology · 2021Article
- Phospholipase pPLAIIIα Increases Germination Rate and Resistance to Turnip Crinkle Virus when Overexpressed.Plant physiology · 2020Article
- Lipid Metabolism in Plants.Plants (Basel, Switzerland) · 2020Article
- Patatin-Related PhospholipasePlants (Basel, Switzerland) · 2020Article
- Overexpression of ginseng cytochrome P450Journal of ginseng research · 2019Article
- Recent Advances in the Cellular and Developmental Biology of Phospholipases in Plants.Frontiers in plant science · 2019Review
- Intracellularly Localized PIN-FORMED8 Promotes Lateral Root Emergence inFrontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phospholipase A(2) (PLA(2)), which hydrolyzes a fatty acyl chain of membrane phospholipids, has been implicated in several biological processes in plants. However, its role in intracellular trafficking in plants has yet to be studied. Here, using pharmacological and genetic approaches, the root hair bioassay system, and PIN-FORMED (PIN) auxin efflux transporters as molecular markers, we demonstrate that plant PLA(2)s are required for PIN protein trafficking to the plasma membrane (PM) in the Arabidopsis thaliana root. PLA(2)alpha, a PLA(2) isoform, colocalized with the Golgi marker. Impairments of PLA(2) function by PLA(2)alpha mutation, PLA(2)-RNA interference (RNAi), or PLA(2) inhibitor treatments significantly disrupted the PM localization of PINs, causing internal PIN compartments to form. Conversely, supplementation with lysophosphatidylethanolamine (the PLA(2) hydrolytic product) restored the PM localization of PINs in the pla(2)alpha mutant and the ONO-RS-082-treated seedling. Suppression of PLA(2) activity by the inhibitor promoted accumulation of trans-Golgi network vesicles. Root hair-specific PIN overexpression (PINox) lines grew very short root hairs, most likely due to reduced auxin levels in root hair cells, but PLA(2) inhibitor treatments, PLA(2)alpha mutation, or PLA(2)-RNAi restored the root hair growth of PINox lines by disrupting the PM localization of PINs, thus reducing auxin efflux. These results suggest that PLA(2), likely acting in Golgi-related compartments, modulates the trafficking of PIN proteins.
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