ArticleThe Journal of biological chemistry2010
Mutations altering a structurally conserved loop-helix-loop region of a viral packaging motor change DNA translocation velocity and processivity.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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Who cites it
25 citing papers in PubMed, 34 citations in OpenAlex.
- Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers.European biophysics journal : EBJ · 2026Review
- Methods for Studying Motor-Driven Viral DNA Packaging in Bacteriophages phi29, Lambda, and T4 via Single DNA Molecule Manipulation and Rapid Solution Exchange.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Optical Tweezers to Study Viruses.Sub-cellular biochemistry · 2024Review
- Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers.Frontiers in molecular biosciences · 2021Article
- Function of a viral genome packaging motor from bacteriophage T4 is insensitive to DNA sequence.Nucleic acids research · 2020Article
- Functional Dissection of a Viral DNA Packaging Machine's Walker B Motif.Journal of molecular biology · 2019Article
- Evidence that a catalytic glutamate and an 'Arginine Toggle' act in concert to mediate ATP hydrolysis and mechanochemical coupling in a viral DNA packaging motor.Nucleic acids research · 2019Article
- Altering the speed of a DNA packaging motor from bacteriophage T4.Nucleic acids research · 2017Article
- Physical and Functional Characterization of a Viral Genome Maturation Complex.Biophysical journal · 2017Article
- Walker-A Motif Acts to Coordinate ATP Hydrolysis with Motor Output in Viral DNA Packaging.Journal of molecular biology · 2016Article
- Mechanisms of DNA Packaging by Large Double-Stranded DNA Viruses.Annual review of virology · 2015Review
- Bacteriophage lambda: Early pioneer and still relevant.Virology · 2015Review
- Continuous allosteric regulation of a viral packaging motor by a sensor that detects the density and conformation of packaged DNA.Biophysical journal · 2015Article
- Molecular interactions and residues involved in force generation in the T4 viral DNA packaging motor.Journal of molecular biology · 2014Article
- Evidence for an electrostatic mechanism of force generation by the bacteriophage T4 DNA packaging motor.Nature communications · 2014Article
- Nonequilibrium dynamics and ultraslow relaxation of confined DNA during viral packaging.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Mechanical operation and intersubunit coordination of ring-shaped molecular motors: insights from single-molecule studies.Biophysical journal · 2014Article
- Function and horizontal transfer of the small terminase subunit of the tailed bacteriophage Sf6 DNA packaging nanomotor.Virology · 2013Article
- Dualities in the analysis of phage DNA packaging motors.Bacteriophage · 2012Article
- Molecular motors for DNA translocation in prokaryotes.Current opinion in biotechnology · 2012Review
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
Abstract
Many double-stranded DNA viruses employ ATP-driven motors to translocate their genomes into small, preformed viral capsids against large forces resisting confinement. Here, we show via direct single-molecule measurements that a mutation T194M downstream of the Walker B motif in the phage lambda gpA packaging motor causes an 8-fold reduction in translocation velocity without substantially changing processivity or force dependence, whereas the mutation G212S in the putative C (coupling) motif causes a 3-fold reduction in velocity and a 6-fold reduction in processivity. Meanwhile a T194M pseudorevertant (T194V) showed a near restoration of the wild-type dynamics. Structural comparisons and modeling show that these mutations are in a loop-helix-loop region that positions the key residues of the catalytic motifs, Walker B and C, in the ATPase center and is structurally homologous with analogous regions in chromosome transporters and SF2 RNA helicases. Together with recently published studies of SpoIIIE chromosome transporter and Ded1 RNA helicase mutants, these findings suggest the presence of a structurally conserved region that may be a part of the mechanism that determines motor velocity and processivity in several different types of nucleic acid translocases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.