ArticleBMC biotechnology2010
Stable expression and phenotypic impact of attacin E transgene in orchard grown apple trees over a 12 year period.
Article in BMC biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 49 citations in OpenAlex.
- Phenotyping, genetics, and "-omics" approaches to unravel and introgress enhanced resistance against apple scab (Horticulture research · 2024Article
- Applying knowledge and experience from potato (Frontiers in bioengineering and biotechnology · 2024Article
- Impact of Intron and Retransformation on Transgene Expression in Leaf and Fruit Tissues of Field-Grown Pear Trees.International journal of molecular sciences · 2023Article
- Fruit Characteristics of Transgenic pear (Pyrus communis L.) Trees During Long-Term Field Trials.Plant foods for human nutrition (Dordrecht, Netherlands) · 2023Article
- An Erwinia amylovora inducible promoter for improvement of apple fire blight resistance.Plant cell reports · 2022Article
- Stability of Transgene Inheritance in Progeny of Field-Grown Pear Trees over a 7-Year Period.Plants (Basel, Switzerland) · 2022Article
- The Arabidopsis pattern recognition receptor EFR enhances fire blight resistance in apple.Horticulture research · 2021Article
- The Rooting of Stem Cuttings and the Stability ofPlants (Basel, Switzerland) · 2019Article
- Overexpression of Pear (Molecules (Basel, Switzerland) · 2019Article
- Biotechnology and apple breeding in Japan.Breeding science · 2016Review
- Cisgenic apple trees; development, characterization, and performance.Frontiers in plant science · 2015Article
- Genomic stability and long-term transgene expression in poplar.Transgenic research · 2013Article
- Pivoting the plant immune system from dissection to deployment.Science (New York, N.Y.) · 2013Review
- Field performance of transgenic citrus trees: assessment of the long-term expression of uidA and nptII transgenes and its impact on relevant agronomic and phenotypic characteristics.BMC biotechnology · 2012Article
- Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines.Transgenic research · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTransgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. The future use of transformed trees on a commercial basis depends upon thorough evaluation of the potential environmental and public health risk of the modified plants, transgene stability over a prolonged period of time and the effect of the gene on tree and fruit characteristics. We studied the stability of expression and the effect on resistance to the fire blight disease of the lytic protein gene, attacin E, in the apple cultivar 'Galaxy' grown in the field for 12 years.
resultsUsing Southern and western blot analysis, we compared transgene copy number and observed stability of expression of this gene in the leaves and fruit in several transformed lines during a 12 year period. No silenced transgenic plant was detected. Also the expression of this gene resulted in an increase in resistance to fire blight throughout 12 years of orchard trial and did not affect fruit shape, size, acidity, firmness, weight or sugar level, tree morphology, leaf shape or flower morphology or color compared to the control.
conclusionOverall, these results suggest that transgene expression in perennial species, such as fruit trees, remains stable in time and space, over extended periods and in different organs. This report shows that it is possible to improve a desirable trait in apple, such as the resistance to a pathogen, through genetic engineering, without adverse alteration of fruit characteristics and tree shape.
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