ArticlePloS one2010
Taqman real-time PCR detects Avipoxvirus DNA in blood of Hawai'i 'amakihi (Hemignathus virens).
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 19 citations in OpenAlex.
- A historical HawaiianiScience · 2025Article
- Genomic associations with poxvirus across divergent island populations in Berthelot's pipit.Molecular ecology · 2022Article
- TaqMan quantitative real-time PCR for detecting Avipoxvirus DNA in various sample types from hummingbirds.PloS one · 2020Article
- Pock forming ability of fowl pox virus isolated from layer chicken and its adaptation in chicken embryo fibroblast cell culture.Veterinary world · 2015Article
- Worldwide phylogenetic relationship of avian poxviruses.Journal of virology · 2013Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundAvipoxvirus sp. is a significant threat to endemic bird populations on several groups of islands worldwide, including Hawai'i, the Galapagos Islands, and the Canary Islands. Accurate identification and genotyping of Avipoxvirus is critical to the study of this disease and how it interacts with other pathogens, but currently available methods rely on invasive sampling of pox-like lesions and may be especially harmful in smaller birds. METHODOLOGY/PRINCIPAL
findingsHere, we present a nested TaqMan Real-Time PCR for the detection of the Avipoxvirus 4b core protein gene in archived blood samples from Hawaiian birds. The method was successful in amplifying Avipoxvirus DNA from packed blood cells of one of seven Hawaiian honeycreepers with confirmed Avipoxvirus infections and 13 of 28 Hawai'i 'amakihi (Hemignathus virens) with suspected Avipoxvirus infections based on the presence of pox-like lesions. Mixed genotype infections have not previously been documented in Hawai'i but were observed in two individuals in this study. CONCLUSIONS/SIGNIFICANCE: We anticipate that this method will be applicable to other closely related strains of Avipoxvirus and will become an important and useful tool in global studies of the epidemiology of Avipoxvirus.
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