ArticleDNA research : an international journal for rapid publication of reports on genes and genomes2010
High-resolution characterization of Toxoplasma gondii transcriptome with a massive parallel sequencing method.
Article in DNA research : an international journal for rapid publication of reports on genes and genomes, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Article
- Genomic insights into host and parasite interactions during intracellular infection by Toxoplasma gondii.PloS one · 2022Article
- High-Resolution Mapping of Transcription Initiation in the Asexual Stages ofFrontiers in cellular and infection microbiology · 2020Article
- toxoMine: an integrated omics data warehouse for Toxoplasma gondii systems biology research.Database : the journal of biological databases and curation · 2015Article
- The Babesia bovis gene and promoter model: an update from full-length EST analysis.BMC genomics · 2014Article
- Transcript maturation in apicomplexan parasites.Current opinion in microbiology · 2014Review
- Lysine acetyltransferase GCN5b interacts with AP2 factors and is required for Toxoplasma gondii proliferation.PLoS pathogens · 2014Article
- A GCN2-like eukaryotic initiation factor 2 kinase increases the viability of extracellular Toxoplasma gondii parasites.Eukaryotic cell · 2011Article
Corrections and comments
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Authors and funding
12 authors.
Funding
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Abstract
For the last couple of years, a method that permits the collection of precise positional information of transcriptional start sites (TSSs) together with digital information of the gene-expression levels in a high-throughput manner was established. We applied this novel method, 'tss-seq', to elucidate the transcriptome of tachyzoites of the Toxoplasma gondii, which resulted in the identification of 124,000 TSSs, and they were clustered into 10,000 transcription regions (TRs) with a statistics-based analysis. The TRs and annotated ORFs were paired, resulting in the identification of 30% of the TRs and 40% of the ORFs without their counterparts, which predicted undiscovered genes and stage-specific transcriptions, respectively. The massive data for TSSs make it possible to execute the first systematic analysis of the T. gondii core promoter structure, and the information showed that T. gondii utilized an initiator-like motif for their transcription in the major and novel motif, the downstream thymidine cluster, which was similar to the Y patch observed in plants. This encyclopaedic analysis also suggested that the TATA box, and the other well-known core promoter elements were hardly utilized.
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Registered trials
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