ArticleThe ISME journal2010
Shifts in the host range of a promiscuous plasmid through parallel evolution of its replication initiation protein.
Article in The ISME journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed, 111 citations in OpenAlex.
- Epistasis and co-adaptation in bacterial genome evolution.Nature reviews. Genetics · 2026Review
- Using auxotrophic donor strains to explore pQBR57 plasmid host range among environmental soil bacterial isolates.Microbiology (Reading, England) · 2026Article
- Modelling the role of the microbiome in antimicrobial resistance across scales.Nature microbiology · 2026Review
- The interplay between ecological networks drives host-plasmid community dynamics.PLoS computational biology · 2026Article
- Not So Mosaic After All? The Core Genomes of IncP-1 Plasmids Evolve Predominantly Through Vertical Transmission.bioRxiv : the preprint server for biology · 2025Article
- Plasmid copy number as a modulator in bacterial pathogenesis and antibiotic resistance.npj antimicrobials and resistance · 2025Review
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.Molecular biology and evolution · 2025Article
- Fitness and adaptive evolution of a Rhodococcus sp. harboring dioxin-catabolic plasmids.World journal of microbiology & biotechnology · 2025Article
- Porin deficiency or plasmid copy number increase mediated carbapenem-resistantEmerging microbes & infections · 2024Article
- Compensatory evolution of chromosomes and plasmids counteracts the plasmid fitness cost.Ecology and evolution · 2024Article
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.bioRxiv : the preprint server for biology · 2024Article
- Diverse plasmid systems and their ecology across human gut metagenomes revealed by PlasX and MobMess.Nature microbiology · 2024Article
- Article
- A mathematician's guide to plasmids: an introduction to plasmid biology for modellers.Microbiology (Reading, England) · 2023Review
- Article
- Compensatory mutations reducing the fitness cost of plasmid carriage occur in plant rhizosphere communities.FEMS microbiology ecology · 2023Article
- High-throughput genetic engineering of nonmodel and undomesticated bacteria via iterative site-specific genome integration.Science advances · 2023Article
- Factors associated with plasmid antibiotic resistance gene carriage revealed using large-scale multivariable analysis.Scientific reports · 2023Article
- Article
- Biofilms preserve the transmissibility of a multi-drug resistance plasmid.NPJ biofilms and microbiomes · 2022Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
Abstract
The ability of bacterial plasmids to adapt to novel hosts and thereby shift their host range is key to their long-term persistence in bacterial communities. Promiscuous plasmids of the incompatibility group P (IncP)-1 can colonize a wide range of hosts, but it is not known if and how they can contract, shift or further expand their host range. To understand the evolutionary mechanisms of host range shifts of IncP-1 plasmids, an IncP-1β mini-replicon was experimentally evolved in four hosts in which it was initially unstable. After 1000 generations in serial batch cultures under antibiotic selection for plasmid maintenance (kanamycin resistance), the stability of the mini-plasmid dramatically improved in all coevolved hosts. However, only plasmids evolved in Shewanella oneidensis showed improved stability in the ancestor, indicating that adaptive mutations had occurred in the plasmid itself. Complete genome sequence analysis of nine independently evolved plasmids showed seven unique plasmid genotypes that had various kinds of single mutations at one locus, namely, the N-terminal region of the replication initiation protein TrfA. Such parallel evolution indicates that this region was under strong selection. In five of the seven evolved plasmids, these trfA mutations resulted in a significantly higher plasmid copy number. Evolved plasmids were found to be stable in four other naive hosts, but could no longer replicate in Pseudomonas aeruginosa. This study shows that plasmids can specialize to a novel host through trade-offs between improved stability in the new host and the ability to replicate in a previously permissive host.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.