Evidence map›Paper›PMID 20519959›Full record

ArticleCell cycle (Georgetown, Tex.)2010

S. pombe genome deletion project: an update.

Mario Spirek, Zsigmond Benko, Martina Carnecka, Cornelia Rumpf, Lubos Cipak, Monika Batova, Ivana Marova, Miyoung Nam, Dong-Uk Kim, Han-Oh Park and 4 more

Open access · hybridAbstract read
In one paragraph

Article in Cell cycle (Georgetown, Tex.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 34 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. PomBase: The Scientific Resource for Fission Yeast.Methods in molecular biology (Clifton, N.J.) · 2018
    Review
  11. Article
  12. Article
  13. Article
  14. Applications of comparative evolution to human disease genetics.Current opinion in genetics & development · 2015
    Review
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 2 countries.

Mario SpirekMax F. Perutz Laboratories, University of Vienna, Vienna, Austria.
Zsigmond Benko
Martina Carnecka
Cornelia Rumpf
Lubos Cipak
Monika Batova
Ivana Marova
Miyoung Nam
Dong-Uk Kim
Han-Oh Park
Jacqueline Hayles
Kwang-Lae Hoe
Paul Nurse
Juraj Gregan
Russian State Agrarian Correspondence University · RUUniversity of Vienna · AT

Funding

Austrian Science Fund FWF F 3403Austrian Science Fund FWF P 18955Austrian Science Fund FWF P 20444Wellcome Trust 093917
6 · The paper itself

Abstract

The fission yeast Schizosaccharomyces pombe is a model organism used widely to study various aspects of eukaryotic biology. A collection of heterozygous diploid strains containing individual deletions in nearly all S. pombe genes has been created using a PCR based strategy. However, deletion of some genes has not been possible using this methodology. Here we use an efficient knockout strategy based on plasmids that contain large regions homologous to the target gene to delete an additional 29 genes. The collection of deletion mutants now covers 99% of the fission yeast open reading frames.

Indexed as

Gene DeletionGenome, FungalDNA Restriction EnzymesGene Knockout TechniquesGenetic VectorsOpen Reading FramesPlasmidsPolymerase Chain ReactionSchizosaccharomycesDNA Restriction Enzymes

Identifiers

PMID20519959
PMCPMC3132452
OpenAlexW2141290892

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.