ArticleChannels (Austin, Tex.)
Ca2+-activated K channels in parotid acinar cells: The functional basis for the hyperpolarized activation of BK channels.
Article in Channels (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Review
- Structural and functional analysis of salivary intercalated duct cells reveals a secretory phenotype.The Journal of physiology · 2023Article
- Article
- Highly localized intracellular CaeLife · 2021Article
- Regulation of BK Channels by Beta and Gamma Subunits.Annual review of physiology · 2019Review
- New saliva secretion model based on the expression of NaPflugers Archiv : European journal of physiology · 2018Article
- Knockout of the LRRC26 subunit reveals a primary role of LRRC26-containing BK channels in secretory epithelial cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Ca²⁺-dependent K⁺ channels in exocrine salivary glands.Cell calcium · 2014Review
- Involvement of large conductance Ca(2+)-activated K (+) channel in laminar shear stress-induced inhibition of vascular smooth muscle cell proliferation.Pflugers Archiv : European journal of physiology · 2013Article
- BK potassium channel modulation by leucine-rich repeat-containing proteins.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Apical Ca2+-activated potassium channels in mouse parotid acinar cells.The Journal of general physiology · 2012Article
- Regulation of Ca²⁺ release through inositol 1,4,5-trisphosphate receptors by adenine nucleotides in parotid acinar cells.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- The LRRC26 protein selectively alters the efficacy of BK channel activators.Molecular pharmacology · 2012Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Fluid secretion relies on a close interplay between Ca(2+)-activated Cl and K channels. Salivary acinar cells contain both large conductance, BK, and intermediate conductance, IK1, K channels. Physiological fluid secretion occurs with only modest (<500 nM) increases in intracellular Ca(2+) levels but BK channels in many cell types and in heterologous expression systems require very high concentrations for significant activation. We report here our efforts to understand this apparent contradiction. We determined the Ca(2+) dependence of IK1 and BK channels in mouse parotid acinar cells. IK1 channels activated with an apparent Ca(2+) affinity of about 350 nM and a Hill coefficient near 3. Native parotid BK channels activated at similar Ca(2+) levels unlike the BK channels in other cell types. Since the parotid BK channel is encoded by an uncommon splice variant, we examined this clone in a heterologous expression system. In contrast to the native parotid channel, activation of this expressed "parSlo" channel required very high levels of Ca(2+). In order to understand the functional basis for the special properties of the native channels, we analyzed the parotid BK channel in the context of the Horrigan-Aldrich model of BK channel gating. We found that the shifted activation of parotid BK channels resulted from a hyperpolarizing shift of the voltage dependence of voltage sensor activation and channel opening and included a large change in the coupling of these two processes.
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