ArticleJournal of dental research2010
Analyzing endodontic infections by deep coverage pyrosequencing.
Article in Journal of dental research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 116 citations in OpenAlex.
- Trial
- Distinct Microbiota and Functional Pathway Profiles Define Success and Failure in Regenerative Endodontic Treatment.International endodontic journal · 2026Observational
- Pulpitis as a microbial disease: single-cell insights into host responses and diagnostic biomarkers for vital pulp therapy.Journal of oral microbiology · 2026Review
- A polymicrobial perspective into the ecological role of Enterococcus faecalis in dental root canal infections.NPJ biofilms and microbiomes · 2025Review
- Article
- Clinical Oral Condition Analysis and the Influence of Highly Active Antiretroviral Therapy on Human Salivary Microbial Community Diversity in HIV-Infected/AIDS Patients.Frontiers in cellular and infection microbiology · 2022Article
- Alterations of bacterial communities of vocal cord mucous membrane increases the risk for glottic laryngeal squamous cell carcinoma.Journal of Cancer · 2021Article
- Comparative Analysis of Bacterial Communities inMicroorganisms · 2020Article
- Analysis of bacterial communities of infected primary teeth in a Mexican population.Medicina oral, patologia oral y cirugia bucal · 2020Article
- In vivo Microbial Diversity Analysis on Different Surfaces of Dental Restorative Materials via 16S rDNA Sequencing.Medical science monitor : international medical journal of experimental and clinical research · 2020Article
- The apical root canal system microbial communities determined by next-generation sequencing.Scientific reports · 2020Article
- Metagenomic investigation of bacteria associated with dental lesions: a cross-sectional study.Medicina oral, patologia oral y cirugia bucal · 2020Article
- The prevalence and impact of lysogeny among oral isolates ofJournal of oral microbiology · 2019Article
- Characterization and Antimicrobial Susceptibility ofDentistry journal · 2018Article
- Apical periodontitis: preliminary assessment of microbiota by 16S rRNA high throughput amplicon target sequencing.BMC oral health · 2018Article
- Illumina MiSeq Sequencing for Preliminary Analysis of Microbiome Causing Primary Endodontic Infections in Egypt.International journal of microbiology · 2018Article
- The mycobiome of root canal infections is correlated to the bacteriome.Clinical oral investigations · 2017Article
- Microbiome of Deep Dentinal Caries Lesions in Teeth with Symptomatic Irreversible Pulpitis.PloS one · 2016Article
- Comparison of Bacterial Community Composition of Primary and Persistent Endodontic Infections Using Pyrosequencing.Journal of endodontics · 2015Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Bacterial diversity in endodontic infections has not been sufficiently studied. The use of modern pyrosequencing technology should allow for more comprehensive analysis than traditional Sanger sequencing. This study investigated bacterial diversity in endodontic infections through taxonomic classification based on 16S rRNA gene sequences generated by 454 GS-FLX pyrosequencing and conventional Sanger capillary sequencing technologies. Sequencings were performed on 7 specimens from endodontic infections. On average, 47 vs. 28,590 sequences were obtained per sample for Sanger sequencing vs. pyrosequencing, representing a 600-fold difference in "depth-of-coverage". Based on Ribosomal Database Project (RDP II) Classifier analysis, pyrosequencing identified 179 bacterial genera in 13 phyla, which was significantly more than Sanger sequencing. The phylum Bacteroidetes was the most prevalent bacterial phylum. These results indicate that bacterial communities in endodontic infections are more diverse than previously demonstrated. In addition, deep-coverage pyrosequencing of the 16S rRNA gene revealed low-abundance micro-organisms with potential clinical implications.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.