ArticleJournal of clinical microbiology2010
Immediate incubation reduces indeterminate results for QuantiFERON-TB Gold in-tube assay.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 3 of them syntheses that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Risk Factors for Indeterminate Interferon-Gamma Release Assay for the Diagnosis of Tuberculosis in Children-A Systematic Review and Meta-Analysis.Frontiers in pediatrics · 2019Pooled it
- Reproducibility of interferon gamma (IFN-γ) release Assays. A systematic review.Annals of the American Thoracic Society · 2014Pooled it
- Pooled it
- Diagnostic accuracy and safety of Cy-Tb for detection of tuberculosis infection: A multicentric study from India.The Indian journal of medical research · 2026Observational
- Host, technical, and environmental factors affecting QuantiFERON-TB Gold In-Tube performance in children below 5 years of age.Scientific reports · 2022Article
- Retrospective Performance Analyses of over Two Million U.S. QuantiFERON Blood Sample Results.Microbiology spectrum · 2021Article
- Development of a Simple and Robust Whole Blood Assay with Dual Co-Stimulation to Quantify the Release of T-Cellular Signature Cytokines in Response toJournal of fungi (Basel, Switzerland) · 2021Article
- Evaluating an Education Program to Reduce Indeterminate QuantiFERON Gold In-Tube Results.Tuberculosis research and treatment · 2018Article
- Performance and variability of QuantiFERON Gold Plus assay associated with phlebotomy type.PloS one · 2018Article
- Indeterminate QuantiFERON-TB Gold Increases Likelihood of Inflammatory Bowel Disease Treatment Delay and Hospitalization.Inflammatory bowel diseases · 2017Article
- Optimization and Interpretation of Serial QuantiFERON Testing to Measure Acquisition of Mycobacterium tuberculosis Infection.American journal of respiratory and critical care medicine · 2017Article
- Serial testing for latent tuberculosis using QuantiFERON-TB Gold In-Tube: A Markov model.Scientific reports · 2016Article
- Interferon Gamma Release Assays for Latent Tuberculosis: What Are the Sources of Variability?Journal of clinical microbiology · 2016Review
- Neutrophil-to-Lymphocyte Ratio Is Associated with Impaired Interferon-Gamma Release to Phytohemagglutinin.PloS one · 2015Article
- High Proportion of Indeterminate QuantiFERON-TB Gold In-Tube Results in an Inpatient Population Is Related to Host Factors and Preanalytical Steps.Open forum infectious diseases · 2014Article
- Extremes of age are associated with indeterminate QuantiFERON-TB gold assay results.Journal of clinical microbiology · 2014Article
- Variability of the QuantiFERON®-TB gold in-tube test using automated and manual methods.PloS one · 2014Article
- Significant reduction in rate of indeterminate results of the QuantiFERON-TB Gold In-Tube test by shortening incubation delay.Journal of clinical microbiology · 2014Article
- Impact of blood volume, tube shaking, and incubation time on reproducibility of QuantiFERON-TB gold in-tube assay.Journal of clinical microbiology · 2013Article
- Effect of prolonged incubation time on results of the QuantiFERON TB gold in-tube assay for diagnosis of latent tuberculosis infection.Clinical and vaccine immunology : CVI · 2013Article
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Authors and funding
5 authors.
Funding
Abstract
In vitro gamma interferon release assays (IGRAs) are increasingly used as an alternative to the traditional tuberculin skin test for the diagnosis of latent Mycobacterium tuberculosis infection. Evaluation of the QuantiFERON-TB Gold in-tube assay (QFT-IT) prior to large-scale implementation at the Stanford Hospital and Clinics for a health care worker screening program revealed a critical preanalytical factor affecting the results. We found that incubation delay significantly increased the frequency of indeterminate results. In this study, QFT-IT was performed with samples from healthy volunteers, and replicate tubes were incubated at 37 degrees C either immediately or after a delay at room temperature for 6 and 12 h. No indeterminate results (0/41) were seen when the assay was performed with immediate incubation. Incubation delays of 6 and 12 h yielded indeterminate results at rates of 10% (2/20) (P = 0.10) and 17.1% (7/41) (P = 0.01), respectively. The increased rate of indeterminate results was due to a decrease in the mean values for the mitogen-nil tubes when incubation was delayed for 6 h (P = 0.004) and 12 h (P < 0.001). The rates of concordance of positive or negative results obtained following immediate incubation and following 6- and 12-h delays were 77.8% (14/18) and 79.4% (27/34), respectively. Subsequent implementation of the immediate incubation procedure in our screening program for 14,830 health care workers yielded an indeterminate result rate of 0.36% over a period of 12 months, a significant improvement over the reported rates of 5 to 40% for QFT-IT. We conclude that immediate incubation of QFT-IT tubes is an effective way to minimize indeterminate results. The effect of incubation delay on the accuracy of QFT-IT remains to be determined.
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