ArticleAmerican journal of physiology. Cell physiology2010
Stretch magnitude and frequency-dependent actin cytoskeleton remodeling in alveolar epithelia.
Article in American journal of physiology. Cell physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Modulation of the hippo-YAP pathway by cyclic stretch in rat type 2 alveolar epithelial cells-a proof-of-concept study.Frontiers in physiology · 2023Article
- Static Stretch Increases the Pro-Inflammatory Response of Rat Type 2 Alveolar Epithelial Cells to Dynamic Stretch.Frontiers in physiology · 2022Article
- The plastic cell: mechanical deformation of cells and tissues.Open biology · 2021Review
- Role of MicroRNA in the Lung's Innate Immune Response.Journal of innate immunity · 2017Review
- Article
- Superoxide mediates tight junction complex dissociation in cyclically stretched lung slices.Journal of biomechanics · 2016Article
- Live Cell Imaging during Mechanical Stretch.Journal of visualized experiments : JoVE · 2015Article
- Investigating cell mechanics with atomic force microscopy.Journal of the Royal Society, Interface · 2015Article
- TREK-1 Regulates Cytokine Secretion from Cultured Human Alveolar Epithelial Cells Independently of Cytoskeletal Rearrangements.PloS one · 2015Article
- Hyperoxia increases the elastic modulus of alveolar epithelial cells through Rho kinase.The FEBS journal · 2014Article
- Rac1 pathway mediates stretch response in pulmonary alveolar epithelial cells.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- Cyclic stretch-induced oxidative stress increases pulmonary alveolar epithelial permeability.American journal of respiratory cell and molecular biology · 2013Article
- Uses of Remnant Human Lung Tissue for Mechanical Stretch Studies.Cellular and molecular bioengineering · 2013Article
- Reproducible uniform equibiaxial stretch of precision-cut lung slices.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- The epidemiological findings and characteristic ground glass appearance on chest CT among patients with blunt lung injury.Journal of emergencies, trauma, and shock · 2012Article
- Rho kinase signaling pathways during stretch in primary alveolar epithelia.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- MicroRNA modulate alveolar epithelial response to cyclic stretch.BMC genomics · 2012Article
- Mechanobiology in lung epithelial cells: measurements, perturbations, and responses.Comprehensive Physiology · 2012Review
- Viscoelastic cell mechanics and actin remodelling are dependent on the rate of applied pressure.PloS one · 2012Article
- Article
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4 authors.
Funding
Abstract
Alveolar epithelial cells (AEC) maintain integrity of the blood-gas barrier with gasket-like intercellular tight junctions (TJ) that are anchored internally to the actin cytoskeleton. We hypothesize that stretch rapidly reorganizes actin (<10 min) into a perijunctional actin ring (PJAR) in a manner that is dependent on magnitude and frequency of the stretch, accompanied by spontaneous movement of actin-anchored receptors at the plasma membrane. Primary AEC monolayers were stretched biaxially to create a change in surface area (DeltaSA) of 12%, 25%, or 37% in a cyclic manner at 0.25 Hz for up to 60 min, or held tonic at 25% DeltaSA for up to 60 min, or left unstretched. By 10 min of stretch PJARs were evident in 25% and 37% DeltaSA at 0.25 Hz, but not for 12% DeltaSA at 0.25 Hz, or at tonic 25% DeltaSA, or with no stretch. Treatment with 1 muM jasplakinolide abolished stretch-induced PJAR formation, however. As a rough index of remodeling rate, we measured spontaneous motions of 5-mum microbeads bound to actin focal adhesion complexes on the apical membrane surfaces; within 1 min of exposure to DeltaSA of 25% and 37%, these motions increased substantially, increased with increasing stretch frequency, and were consistent with our mechanistic hypothesis. With a tonic stretch, however, the spontaneous motion of microbeads attenuated back to unstretched levels, whereas PJAR remained unchanged. Stretch did not increase spontaneous microbead motion in human alveolar epithelial adenocarcinoma A549 monolayers, confirming that this actin remodeling response to stretch was a cell-type specific response. In summary, stretch of primary rat AEC monolayers forms PJARs and rapidly reorganized actin binding sites at the plasma membrane in a manner dependent on stretch magnitude and frequency.
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