ArticleMolecular biology of the cell2010
Phosphorylation controls autoinhibition of cytoplasmic linker protein-170.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 41 citations in OpenAlex.
- CDKL5 regulates the initiation of retrograde axonal transport through CLIP170-dynactin complex formation.The FEBS journal · 2026Article
- Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration.Cell death & disease · 2022Article
- Therapeutic potential of pregnenolone and pregnenolone methyl ether on depressive and CDKL5 deficiency disorders: Focus on microtubule targeting.Journal of neuroendocrinology · 2022Review
- CLIP-170S is a microtubule +TIP variant that confers resistance to taxanes by impairing drug-target engagement.Developmental cell · 2021Article
- Stress-induced phosphorylation of CLIP-170 by JNK promotes microtubule rescue.The Journal of cell biology · 2020Article
- Insulin Induces Microtubule Stabilization and Regulates the Microtubule Plus-end Tracking Protein Network in Adipocytes.Molecular & cellular proteomics : MCP · 2019Article
- CAP-Gly proteins contribute to microtubule-dependent trafficking via interactions with the C-terminal aromatic residue of α-tubulin.Small GTPases · 2019Review
- Mapping multivalency in the CLIP-170-EB1 microtubule plus-end complex.The Journal of biological chemistry · 2019Article
- GTP-binding facilitates EB1 recruitment onto microtubules by relieving its auto-inhibition.Scientific reports · 2018Article
- The impact of cytoskeletal organization on the local regulation of neuronal transport.Nature reviews. Neuroscience · 2017Review
- A role for the yeast CLIP170 ortholog, the plus-end-tracking protein Bik1, and the Rho1 GTPase in Snc1 trafficking.Journal of cell science · 2016Article
- Neurotrophin signaling endosomes: biogenesis, regulation, and functions.Current opinion in neurobiology · 2016Review
- α-Tubulin Tyrosination and CLIP-170 Phosphorylation Regulate the Initiation of Dynein-Driven Transport in Neurons.Cell reports · 2016Article
- Microtubule plus end-associated CLIP-170 initiates HSV-1 retrograde transport in primary human cells.The Journal of cell biology · 2015Article
- Drosophila CLIP-190 and mammalian CLIP-170 display reduced microtubule plus end association in the nervous system.Molecular biology of the cell · 2015Article
- Reconstitution of a hierarchical +TIP interaction network controlling microtubule end tracking of dynein.Nature cell biology · 2014Article
- Article
- Pregnenolone activates CLIP-170 to promote microtubule growth and cell migration.Nature chemical biology · 2013Article
- The actin-microtubule cross-linking activity of Drosophila Short stop is regulated by intramolecular inhibition.Molecular biology of the cell · 2013Article
- Ordered recruitment of dynactin to the microtubule plus-end is required for efficient initiation of retrograde axonal transport.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
Cytoplasmic linker protein (CLIP)-170 is a microtubule (MT) plus-end-tracking protein that regulates MT dynamics and links MT plus ends to different intracellular structures. We have shown previously that intramolecular association between the N and C termini results in autoinhibition of CLIP-170, thus altering its binding to MTs and the dynactin subunit p150(Glued) (J. Cell Biol. 2004: 166, 1003-1014). In this study, we demonstrate that conformational changes in CLIP-170 are regulated by phosphorylation that enhances the affinity between the N- and C-terminal domains. By using site-directed mutagenesis and phosphoproteomic analysis, we mapped the phosphorylation sites in the third serine-rich region of CLIP-170. A phosphorylation-deficient mutant of CLIP-170 displays an "open" conformation and a higher binding affinity for growing MT ends and p150(Glued) as compared with nonmutated protein, whereas a phosphomimetic mutant confined to the "folded back" conformation shows decreased MT association and does not interact with p150(Glued). We conclude that phosphorylation regulates CLIP-170 conformational changes resulting in its autoinhibition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.