Trial reportAmerican journal of physiology. Regulatory, integrative and comparative physiology2010
Muscle protein breakdown has a minor role in the protein anabolic response to essential amino acid and carbohydrate intake following resistance exercise.
Trial report in American journal of physiology. Regulatory, integrative and comparative physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT00891696 (Nutritional and Contractile Regulation of Muscle Growth), which is not on this map. Cited by 86 papers, 4 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Nutritional and Contractile Regulation of Muscle Growth (Cycle 2)
Who cites it
86 citing papers in PubMed, 4 syntheses or guidelines pooled it, 167 citations in OpenAlex.
- The Effect of Carbohydrate Intake on Muscle Hypertrophy: A Systematic Review and Meta-analysis.Sports medicine (Auckland, N.Z.) · 2026Pooled it
- Resistance exercise training improves disuse-induced skeletal muscle atrophy in humans: a meta-analysis of randomized controlled trials.BMC musculoskeletal disorders · 2025Pooled it
- Evaluating the Leucine Trigger Hypothesis to Explain the Post-prandial Regulation of Muscle Protein Synthesis in Young and Older Adults: A Systematic Review.Frontiers in nutrition · 2021Pooled it
- The effects of protein supplements on muscle mass, strength, and aerobic and anaerobic power in healthy adults: a systematic review.Sports medicine (Auckland, N.Z.) · 2015Pooled it
- Ingestion of diverse protein-rich whole-foods result in similar post exercise whole body and myofibrillar protein synthesis rates compared with a more isolated protein source in young adults.The American journal of clinical nutrition · 2026Trial
- Influence of protein source (cricket, pea, whey) on amino acid bioavailability and activation of the mTORC1 signaling pathway after resistance exercise in healthy young males.European journal of nutrition · 2023Trial
- Carbohydrate intake in recovery from aerobic exercise differentiates skeletal muscle microRNA expression.American journal of physiology. Endocrinology and metabolism · 2022Trial
- Effect of eccentric and concentric contraction mode on myogenic regulatory factors expression in human vastus lateralis muscle.Journal of muscle research and cell motility · 2022Trial
- Exercise Mitigates the Loss of Muscle Mass by Attenuating the Activation of Autophagy during Severe Energy Deficit.Nutrients · 2019Trial
- Blood Flow Restriction Only Increases Myofibrillar Protein Synthesis with Exercise.Medicine and science in sports and exercise · 2019Trial
- Native Whey Induces Similar Post Exercise Muscle Anabolic Responses as Regular Whey, Despite Greater Leucinemia, in Elderly Individuals.The journal of nutrition, health & aging · 2019Trial
- PI3K-AKT-FOXO1 pathway targeted by skeletal muscle microRNA to suppress proteolytic gene expression in response to carbohydrate intake during aerobic exercise.Physiological reports · 2018Trial
- Effects of Whey Protein Hydrolysate Ingestion on Postprandial Aminoacidemia Compared with a Free Amino Acid Mixture in Young Men.Nutrients · 2018Trial
- Impact of resistance training on the autophagy-inflammation-apoptosis crosstalk in elderly subjects.Aging · 2017Trial
- Native whey protein with high levels of leucine results in similar post-exercise muscular anabolic responses as regular whey protein: a randomized controlled trial.Journal of the International Society of Sports Nutrition · 2017Trial
- Minimal dose of milk protein concentrate to enhance the anabolic signalling response to a single bout of resistance exercise; a randomised controlled trial.Journal of the International Society of Sports Nutrition · 2017Trial
- Fish oil supplementation suppresses resistance exercise and feeding-induced increases in anabolic signaling without affecting myofibrillar protein synthesis in young men.Physiological reports · 2016Trial
- The anabolic response to a meal containing different amounts of protein is not limited by the maximal stimulation of protein synthesis in healthy young adults.American journal of physiology. Endocrinology and metabolism · 2016Trial
- Predictors of muscle protein synthesis after severe pediatric burns.The journal of trauma and acute care surgery · 2015Trial
- How Acute and Chronic Exercise Regulate Muscle Atrophic Genes to Mitigate Sarcopenia: A Narrative Review.Sports medicine (Auckland, N.Z.) · 2026Review
26 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Muscle protein breakdown (MPB) is increased following resistance exercise, but ingestion of carbohydrate during postexercise recovery can decrease MPB with no effect on muscle protein synthesis (MPS). We sought to determine whether a combination of essential amino acids (EAA) with low carbohydrate or high carbohydrate could effectively reduce MPB following resistance exercise and improve muscle protein net balance (NB). We hypothesized that higher levels of carbohydrate and resulting increases in circulating insulin would inhibit MPB and associated signaling, resulting in augmented NB. Thirteen male subjects were assigned to one of two groups receiving equivalent amounts of EAA (approximately 20 g) but differing carbohydrate levels (low = 30, high = 90 g). Groups ingested nutrients 1 h after an acute bout of leg resistance exercise. Leg phenylalanine kinetics (e.g., MPB, MPS, NB), signaling proteins, and mRNA expression were assessed on successive muscle biopsies using stable isotopic techniques, immunoblotting, and real-time quantitative PCR, respectively. MPB tended to decrease (P < 0.1) and MPS increased (P < 0.05) similarly in both groups following nutrient ingestion. No group differences were observed, but muscle ring finger 1 (MuRF1) protein content and MuRF1 mRNA expression increased following resistance exercise and remained elevated following nutrient ingestion, while autophagy marker (light-chain 3B-II) decreased after nutrient ingestion (P < 0.05). Forkhead box-O3a phosphorylation, total muscle atrophy F-box (MAFbx) protein, and MAFbx and caspase-3 mRNA expression were unchanged. We conclude that the enhanced muscle protein anabolic response detected when EAA+carbohydrate are ingested postresistance exercise is primarily due to an increase in MPS with minor changes in MPB, regardless of carbohydrate dose or circulating insulin level.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.