ArticleJournal of experimental botany2010
Tissue-specific transcriptome profiling of the citrus fruit epidermis and subepidermis using laser capture microdissection.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 68 citations in OpenAlex.
- Developing a simple and rapid method for cell-specific transcriptome analysis through laser microdissection: insights from citrus rind with broader implications.Plant methods · 2024Article
- A novel immunohistochemical protocol for paraffin embedded tissue sections using free-floating techniques.Frontiers in neuroanatomy · 2023Article
- Laser Microdissection: A High-Precision Approach to Isolate Specific Cell Types from Any Plant Species for Downstream Molecular Analyses.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Leaf layer-based transcriptome profiling for discovery of epidermal-selective promoters in Medicago truncatula.Planta · 2022Article
- Molecular Biology, Composition and Physiological Functions of Cuticle Lipids in Fleshy Fruits.Plants (Basel, Switzerland) · 2022Review
- Laser Microdissection of Specific Stem-Base Tissue Types from Olive Microcuttings for Isolation of High-Quality RNA.Biology · 2021Article
- Long-Term Impact of Chemical and Alternative Fungicides Applied to GrapevineInternational journal of molecular sciences · 2020Article
- Genome-Wide Association Study for Maize Leaf Cuticular Conductance Identifies Candidate Genes Involved in the Regulation of Cuticle Development.G3 (Bethesda, Md.) · 2020Article
- Identification of the Virulence Factors ofInternational journal of molecular sciences · 2019Article
- QTL and Transcriptomic Analyses Implicate Cuticle Transcription FactorFrontiers in plant science · 2019Article
- A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms.Plant methods · 2019Article
- Cell Wall Remodeling in Abscission Zone Cells during Ethylene-Promoted Fruit Abscission in Citrus.Frontiers in plant science · 2017Article
- Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling.Nature protocols · 2016Article
- Tissue-specific laser microdissection of the Brassica napus funiculus improves gene discovery and spatial identification of biological processes.Journal of experimental botany · 2016Article
- Pigmentation in sand pear (Pyrus pyrifolia) fruit: biochemical characterization, gene discovery and expression analysis with exocarp pigmentation mutant.Plant molecular biology · 2014Article
- Transcriptional dynamics of the developing sweet cherry (Prunus avium L.) fruit: sequencing, annotation and expression profiling of exocarp-associated genes.Horticulture research · 2014Article
- Article
- Using a periclinal chimera to unravel layer-specific gene expression in plants.The Plant journal : for cell and molecular biology · 2013Article
- Functional genomics of a generalist parasitic plant: laser microdissection of host-parasite interface reveals host-specific patterns of parasite gene expression.BMC plant biology · 2013Article
- Optimizing Frozen Sample Preparation for Laser Microdissection: Assessment of CryoJane Tape-Transfer System®.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most studies of the biochemical and regulatory pathways that are associated with, and control, fruit expansion and ripening are based on homogenized bulk tissues, and do not take into consideration the multiplicity of different cell types from which the analytes, be they transcripts, proteins or metabolites, are extracted. Consequently, potentially valuable spatial information is lost and the lower abundance cellular components that are expressed only in certain cell types can be diluted below the level of detection. In this study, laser microdissection (LMD) was used to isolate epidermal and subepidermal cells from green, expanding Citrus clementina fruit and their transcriptomes were compared using a 20k citrus cDNA microarray and quantitative real-time PCR. The results show striking differences in gene expression profiles between the two cell types, revealing specific metabolic pathways that can be related to their respective organelle composition and cell wall specialization. Microscopy provided additional evidence of tissue specialization that could be associated with the transcript profiles with distinct differences in organelle and metabolite accumulation. Subepidermis predominant genes are primarily involved in photosynthesis- and energy-related processes, as well as cell wall biosynthesis and restructuring. By contrast, the most epidermis predominant genes are related to the biosynthesis of the cuticle, flavonoids, and defence responses. Furthermore, the epidermis transcript profile showed a high proportion of genes with no known function, supporting the original hypothesis that analysis at the tissue/cell specific levels can promote gene discovery and lead to a better understanding of the specialized contribution of each tissue to fruit physiology.
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