ArticleOptics letters2010
Fully linear reconstruction method for fluorescence yield and lifetime through inverse complex-source formulation: simulation studies.
Article in Optics letters, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
In fluorescence imaging, both fluorescence yield and lifetime are of great importance. Traditionally, with the frequency-domain data, two parameters can be directly recovered through a nonlinear formulation. However, the reconstruction accuracy highly depends on initial guesses. To overcome this hurdle, we propose the linear scheme via an inverse complex-source formulation. Using the real and imaginary parts of the frequency-domain data, the proposed method is fully linear; it is not sensitive to initial guesses and is stable with high-level noise. Meanwhile, the algorithm is efficient, and the reconstruction takes one or a few iterations. In addition, the colocalization constraint due to the unique feature of fluorescence imaging is imposed to enhance algorithm performance. The algorithms are tested with simulated data.
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