ArticleNanotechnology2010
Monitoring ligand-receptor interactions by photonic force microscopy.
Article in Nanotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- High-frequency micro-viscoelasticity of biofluids by using optical trapping interferometry.Biophysical reviews · 2026Review
- Rouse and Zimm Short-Time Exponents When Subtracting the Solvent Contribution in Semidilute Polymeric Solutions.Macromolecules · 2025Article
- ATP-induced reconfiguration of the micro-viscoelasticity of cardiac and skeletal myosin solutions.Applied physics letters · 2024Article
- FimH-mannose noncovalent bonds survive minutes to hours under force.Biophysical journal · 2024Article
- Measuring Stepwise Binding of Thermally Fluctuating Particles to Cell Membranes without Fluorescence.Biophysical journal · 2020Article
- Heterogeneous and rate-dependent streptavidin-biotin unbinding revealed by high-speed force spectroscopy and atomistic simulations.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Selective transport control on molecular velcro made from intrinsically disordered proteins.Nature nanotechnology · 2014Article
- Force mapping during the formation and maturation of cell adhesion sites with multiple optical tweezers.PloS one · 2013Article
- Optical trapping microrheology in cultured human cells.The European physical journal. E, Soft matter · 2012Article
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Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We introduce a method for the acquisition of single molecule force measurements of ligand-receptor interactions using the photonic force microscope (PFM). Biotin-functionalized beads, manipulated with an optical trap, and a streptavidin-functionalized coverslip were used to measure the effect of different pulling forces on the lifetime of individual streptavidin-biotin complexes. By optimizing the design of the optical trap and selection of the appropriate bead size, pulling forces in excess of 50 pN were achieved. Based on the amplitude of three-dimensional (3D) thermal position fluctuations of the attached bead, we were able to select for a bead-coverslip interaction that was mediated by a single streptavidin-biotin complex. Moreover, the developed experimental system was greatly accelerated by automation of data acquisition and analysis. In force-dependent kinetic measurements carried out between streptavidin and biotin, we observed that the streptavidin-biotin complex exhibited properties of a catch bond, with the lifetime increasing tenfold when the pulling force increased from 10 to 20 pN. We also show that silica beads were more appropriate than polystyrene beads for the force measurements, as tethers, longer than 200 nm, could be extracted from polystyrene beads.
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Registered trials
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