ArticleGenetics2010
The gypsy insulator of Drosophila melanogaster, together with its binding protein suppressor of Hairy-wing, facilitate high and precise expression of transgenes in Arabidopsis thaliana.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 45 citations in OpenAlex.
- Configuring the Code: Enhancer-Promoter Arrangement and Transcriptional Regulation.Journal of molecular biology · 2026Review
- An RbohC and RbohE-mediated ROS oscillatory circuit regulates root cap turnover in Arabidopsis.iScience · 2025Article
- Small DNA elements can act as both insulators and silencers in plants.The Plant cell · 2025Article
- Potassium Chloroaurate-MediatedNanomaterials (Basel, Switzerland) · 2022Article
- You shall not pass! A Chromatin barrier story in plants.Frontiers in plant science · 2022Review
- Exploiting a Y chromosome-linked Cas9 for sex selection and gene drive.Nature communications · 2021Article
- Review
- Mutational analysis identifies functional Rap1, Su(Hw), and CTCF insulator sites in Arabidopsis thaliana.Plant cell reports · 2020Article
- The SAUR41 subfamily of SMALL AUXIN UP RNA genes is abscisic acid inducible to modulate cell expansion and salt tolerance in Arabidopsis thaliana seedlings.Annals of botany · 2020Article
- Biosystems Design to Accelerate CBiodesign research · 2020Review
- Reconfiguring Plant Metabolism for Biodegradable Plastic Production.Biodesign research · 2020Review
- The Effects of Transcription Directions of Transgenes and the gypsy Insulators on the Transcript Levels of Transgenes in Transgenic Arabidopsis.Scientific reports · 2017Article
- Control of vein network topology by auxin transport.BMC biology · 2015Article
- Evolutionary transgenomics: prospects and challenges.Frontiers in plant science · 2015Article
- Expanding the roles of chromatin insulators in nuclear architecture, chromatin organization and genome function.Cellular and molecular life sciences : CMLS · 2014Review
- Maize transformation technology development for commercial event generation.Frontiers in plant science · 2014Review
- Specific expression of DR5 promoter in rice roots using a tCUP derived promoter-reporter system.PloS one · 2014Article
- The tissue-specific and developmentally regulated expression patterns of the SAUR41 subfamily of small auxin up RNA genes: potential implications.Plant signaling & behavior · 2013Article
- Exogenous gypsy insulator sequences modulate transgene expression in the malaria vector mosquito, Anopheles stephensi.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The variation of expression pattern exhibited by a transgene as a result of random integration, known as position effect, is, among other mechanisms, a particular challenge to reverse genetics. We present a strategy to counteract position effect in Arabidopsis thaliana by flanking the transgenes with the gypsy insulator from Drosophila melanogaster. In addition, Suppressor of Hairy-wing [Su(Hw)], the binding protein of the gypsy insulator, was coexpressed. Results indicated that the gypsy insulators could efficiently improve the expression levels of reporter genes driven by various kinds of promoters by 8- to 13-fold. Coexpression of the Su(Hw) protein led to a more uniform expression level of transgenes, as the coefficient of variation of expression levels was reduced further. The gypsy-Su(Hw) system enhanced expression levels, but did not alter the specificity of promoter activities, as experimentally evidenced by the promoters of the PIN and the AFB gene families. Interestingly, the gypsy insulator was also able to improve the expression of a selectable marker gene outside the insulated region, which facilitated the screen of transformants. Our system will likely decrease the number of lines that experimenters need to create and examine for a given transgene by contributing to relatively high and precise expression of transgenes in plants. Certain features of the gypsy insulator in Arabidopsis also provide new perspectives on the insulator field.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.