ArticleThe Journal of biological chemistry2010
Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse array of plant proteolytic complexes.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
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Who cites it
81 citing papers in PubMed.
- Ubiquitination-driven proteostasis remodeling underlies oxidative stress and actin degradation in citrus huanglongbing.Plant physiology · 2026Article
- The Nuclear Interactome of ATR7 Implicates a Chromatin-Based Repression Mechanism Controlling Oxidative Stress Tolerance and Programmed Cell Death in Arabidopsis.International journal of molecular sciences · 2026Article
- The 26S proteasome engages in priming explants with auxin plus cytokinin for somatic embryogenesis of Coffea canephora.Protoplasma · 2026Article
- The transcription factor ANAC017 links mitochondrial retrograde signaling with the ubiquitin-proteasome system to control mitochondrial function in Arabidopsis.Plant communications · 2026Article
- Catch & Release-rapid cost-effective protein purification from plants using a DIY GFP-Trap-protease approach.The Plant journal : for cell and molecular biology · 2025Article
- Streamline Protocol forPlant direct · 2025Article
- The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis.Nature communications · 2025Article
- Proteasomes accumulate in the plant apoplast where they participate in microbe-associated molecular pattern (MAMP)-triggered pathogen defense.Nature communications · 2025Article
- The Deubiquitinating Enzyme AMSH1 Contributes to Plant Immunity Through Regulating the Stability of BDA1.Plants (Basel, Switzerland) · 2025Article
- The Structural Role of RPN10 in the 26S Proteasome and an RPN2-Binding Residue on RPN13 Are Functionally Important in Arabidopsis.International journal of molecular sciences · 2024Article
- 40S Ribosomal protein S6 kinase integrates daylength perception and growth regulation in Arabidopsis thaliana.Plant physiology · 2024Article
- Contrasting Impacts of Ubiquitin Overexpression on Arabidopsis Growth and Development.Plants (Basel, Switzerland) · 2024Article
- The chromatin remodeller MdRAD5B enhances drought tolerance by coupling MdLHP1-mediated H3K27me3 in apple.Plant biotechnology journal · 2024Article
- Protein disulfide isomerase-9 interacts with the lumenal region of the transmembrane endoplasmic reticulum stress sensor kinase, IRE1, to modulate the unfolded protein response inFrontiers in plant science · 2024Article
- Article
- COG-imposed Golgi functional integrity determines the onset of dark-induced senescence.Nature plants · 2023Article
- The Ubiquitin-26S Proteasome System-A Versatile Player Worthy of Close Attention in Plants.International journal of molecular sciences · 2023Article
- HEAT SHOCK PROTEIN 90.6 interacts with carbon and nitrogen metabolism components during seed development.Plant physiology · 2023Article
- Plant deubiquitinases: from structure and activity to biological functions.Plant cell reports · 2023Review
- To Kill or to Be Killed: How Does the Battle between the UPS and Autophagy Maintain the Intracellular Homeostasis in Eukaryotes?International journal of molecular sciences · 2023Review
21 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
Abstract
Selective proteolysis in plants is largely mediated by the ubiquitin (Ub)/proteasome system in which substrates, marked by the covalent attachment of Ub, are degraded by the 26 S proteasome. The 26 S proteasome is composed of two subparticles, the 20 S core protease (CP) that compartmentalizes the protease active sites and the 19 S regulatory particle that recognizes and translocates appropriate substrates into the CP lumen for breakdown. Here, we describe an affinity method to rapidly purify epitope-tagged 26 S proteasomes intact from Arabidopsis thaliana. In-depth mass spectrometric analyses of preparations generated from young seedlings confirmed that the 2.5-MDa CP-regulatory particle complex is actually a heterogeneous set of particles assembled with paralogous pairs for most subunits. A number of these subunits are modified post-translationally by proteolytic processing, acetylation, and/or ubiquitylation. Several proteasome-associated proteins were also identified that likely assist in complex assembly and regulation. In addition, we detected a particle consisting of the CP capped by the single subunit PA200 activator that may be involved in Ub-independent protein breakdown. Taken together, it appears that a diverse and highly dynamic population of proteasomes is assembled in plants, which may expand the target specificity and functions of intracellular proteolysis.
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