ArticleZebrafish2010
Determination of sperm concentration for small-bodied biomedical model fishes by use of microspectrophotometry.
Article in Zebrafish, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Is catalase an effective additive to alleviate oxidative stress during cryopreservation of zebrafish sperm at the repository level?Cryobiology · 2022Article
- Cryopreservation of Pooled Sperm Samples.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Changes to Extender, Cryoprotective Medium, and In Vitro Fertilization Improve Zebrafish Sperm Cryopreservation.Zebrafish · 2018Article
- Quality evaluation of sperm from livebearing fishes: Standardized assessment of sperm bundles (spermatozeugmata) from Xenotoca eiseni (Goodeidae).Theriogenology · 2018Article
- Challenges in Development of Sperm Repositories for Biomedical Fishes: Quality Control in Small-Bodied Species.Zebrafish · 2017Article
- Cryobanking of aquatic species.Aquaculture (Amsterdam, Netherlands) · 2017Article
- A Procedure-Spanning Analysis of Plasma Membrane Integrity for Assessment of Cell Viability in Sperm Cryopreservation of Zebrafish Danio rerio.Zebrafish · 2016Article
- Determination of sperm concentration using flow cytometry with simultaneous analysis of sperm plasma membrane integrity in zebrafish Danio rerio.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2016Article
- Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation.Biomedical microdevices · 2015Article
- Sources of variation in flow cytometric analysis of aquatic species sperm: The effect of cryoprotectants on flow cytometry scatter plots and subsequent population gating.Aquaculture (Amsterdam, Netherlands) · 2012Article
- Article
- Microfluidic mixing for sperm activation and motility analysis of pearl Danio zebrafish.Theriogenology · 2012Article
- Outlook for development of high-throughput cryopreservation for small-bodied biomedical model fishes.Comparative biochemistry and physiology. Toxicology & pharmacology : CBP · 2011Article
- High-throughput cryopreservation of spermatozoa of blue catfish (Ictalurus furcatus): Establishment of an approach for commercial-scale processing.Cryobiology · 2011Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The goal of this study was to establish an efficient method for determination of sperm concentration requiring only 1-2 microL of sample by use of microspectrophotometry. The objectives were (1) determination of wavelengths with absorbance profiles appropriate for analysis of sperm suspensions from zebrafish Danio rerio, green swordtail Xiphophorus helleri, and medaka Oryzias latipes collected by crushing of dissected testis or by stripping of live males; (2) generation of standard curves and equations between sperm sample absorbance and sperm concentration estimated by hemocytometer counts; (3) accuracy verification of equations for estimating concentration by microspectrophotometry; and (4) analysis of the precision in generating equations and estimation of sperm concentration. Within the visible wavelengths (380-750 nm) there was no single maximal absorbance peak. For zebrafish, a linear correlation was established with an effective absorbance range of 0.034-0.936 for crushed samples, and 0.028-0.961 for stripped samples at 400 nm. For Xiphophorus, the effective absorbance range was 0.014-1.154 for crushed samples, and the effective range was 0.038-1.082 for stripped samples. For medaka, the effective range was 0.041-0.896 for crushed samples. The accuracy of these equations was verified by comparison of sample concentrations counted with hemocytometer and calculated with equations, and no significant differences (p = 0.447) were observed. Measurement of serially diluted aliquots from pooled samples verified the precision of techniques used. Overall, this confirmed that microspectrophotometric estimation of sperm concentration is accurate, efficient, and sample-saving for use with small-bodied fishes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.