ArticleBiophysical journal2010
Mechanisms for the intracellular manipulation of organelles by conventional electroporation.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
38 citing papers in PubMed, 1 citations in OpenAlex.
- Resolving nanosecond kinetics of the optical membrane potential in pulsed electric fields.Bioelectrochemistry (Amsterdam, Netherlands) · 2026Article
- Simulation of electroporation threshold based on the evolution of transmembrane potential and pore density.PeerJ · 2025Article
- Numerical assessments of geometry, proximity and multi-electrode effects on electroporation in mitochondria and the endoplasmic reticulum to nanosecond electric pulses.Scientific reports · 2024Article
- Ex vivo gene editing and cell therapy for hereditary tyrosinemia type 1.Hepatology communications · 2024Article
- Acute ATP loss during irreversible electroporation mediates caspase independent cell death.Bioelectrochemistry (Amsterdam, Netherlands) · 2023Article
- Evaluation of Electrochemotherapy with Bleomycin in the Treatment of Colorectal Hepatic Metastases in a Rat Model.Cancers · 2023Article
- Processing Technologies for the Extraction of Value-Added Bioactive Compounds from Tea.Food engineering reviews · 2023Review
- Gene Electrotransfer into Mammalian Cells Using Commercial Cell Culture Inserts with Porous Substrate.Pharmaceutics · 2022Article
- Nanosecond Pulsed Electric Field (nsPEF): Opening the Biotechnological Pandora's Box.International journal of molecular sciences · 2022Review
- The interplay of excitation and electroporation in nanosecond pulse stimulation.Bioelectrochemistry (Amsterdam, Netherlands) · 2020Review
- Nanosecond pulsed electric fields modulate the expression of the astaxanthin biosynthesis genes psy, crtR-b and bkt 1 in Haematococcus pluvialis.Scientific reports · 2020Article
- Characterization in Dual Activation by Oxaliplatin, a Platinum-Based Chemotherapeutic Agent of Hyperpolarization-Activated Cation and Electroporation-Induced Currents.International journal of molecular sciences · 2020Article
- Confocal Microscopy Improves 3D Microdosimetry Applied to Nanoporation Experiments Targeting Endoplasmic Reticulum.Frontiers in bioengineering and biotechnology · 2020Article
- Excitation and electroporation by MHz bursts of nanosecond stimuli.Biochemical and biophysical research communications · 2019Article
- The second phase of bipolar, nanosecond-range electric pulses determines the electroporation efficiency.Bioelectrochemistry (Amsterdam, Netherlands) · 2018Article
- Influence of Pulsed Electric Fields and Mitochondria-Cytoskeleton Interactions on Cell Respiration.Biophysical journal · 2018Article
- Monoclonal Cell Line Generation and CRISPR/Cas9 Manipulation via Single-Cell Electroporation.Small (Weinheim an der Bergstrasse, Germany) · 2018Article
- Article
- Electropermeabilization of Inner and Outer Cell Membranes with Microsecond Pulsed Electric Fields: Quantitative Study with Calcium Ions.Scientific reports · 2017Article
- Irreversible electroporation inhibits pro-cancer inflammatory signaling in triple negative breast cancer cells.Bioelectrochemistry (Amsterdam, Netherlands) · 2017Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Conventional electroporation (EP) changes both the conductance and molecular permeability of the plasma membrane (PM) of cells and is a standard method for delivering both biologically active and probe molecules of a wide range of sizes into cells. However, the underlying mechanisms at the molecular and cellular levels remain controversial. Here we introduce a mathematical cell model that contains representative organelles (nucleus, endoplasmic reticulum, mitochondria) and includes a dynamic EP model, which describes formation, expansion, contraction, and destruction for the plasma and all organelle membranes. We show that conventional EP provides transient electrical pathways into the cell, sufficient to create significant intracellular fields. This emerging intracellular electrical field is a secondary effect due to EP and can cause transmembrane voltages at the organelles, which are large enough and long enough to gate organelle channels, and even sufficient, at some field strengths, for the poration of organelle membranes. This suggests an alternative to nanosecond pulsed electric fields for intracellular manipulations.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.