ArticleBiophysical journal2010
Synchronization of circadian oscillation of phosphorylation level of KaiC in vitro.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Determining subunit-subunit interaction from statistics of cryo-EM images: observation of nearest-neighbor coupling in a circadian clock protein complex.Nature communications · 2023Article
- Role of the reaction-structure coupling in temperature compensation of the KaiABC circadian rhythm.PLoS computational biology · 2022Article
- Mechanism of autonomous synchronization of the circadian KaiABC rhythm.Scientific reports · 2021Article
- Non-sinusoidal Waveform in Temperature-Compensated Circadian Oscillations.Biophysical journal · 2019Article
- Single-molecular and ensemble-level oscillations of cyanobacterial circadian clock.Biophysics and physicobiology · 2018Article
- Role of ATP Hydrolysis in Cyanobacterial Circadian Oscillator.Scientific reports · 2017Article
- Systems Biology-Derived Discoveries of Intrinsic Clocks.Frontiers in neurology · 2017Review
- Toward Multiscale Models of Cyanobacterial Growth: A Modular Approach.Frontiers in bioengineering and biotechnology · 2016Review
- KaiC intersubunit communication facilitates robustness of circadian rhythms in cyanobacteria.Nature communications · 2013Article
- Structural and dynamic aspects of protein clocks: how can they be so slow and stable?Cellular and molecular life sciences : CMLS · 2012Review
- Comprehensive modelling of the Neurospora circadian clock and its temperature compensation.PLoS computational biology · 2012Article
- Understanding systems-level properties: timely stories from the study of clocks.Nature reviews. Genetics · 2011Review
- The itty-bitty time machine genetics of the cyanobacterial circadian clock.Advances in genetics · 2011Review
- The cyanobacterial circadian system: from biophysics to bioevolution.Annual review of biophysics · 2011Review
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Authors and funding
3 authors.
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Abstract
In recent experimental reports, robust circadian oscillation of the phosphorylation level of KaiC has been reconstituted by incubating three cyanobacterial proteins, KaiA, KaiB, and KaiC, with ATP in vitro. This reconstitution indicates that protein-protein interactions and the associated ATP hydrolysis suffice to generate the oscillation, and suggests that the rhythm arising from this protein-based system is the circadian clock pacemaker in cyanobacteria. The mechanism of this reconstituted oscillation, however, remains elusive. In this study, we extend our previous model of oscillation by explicitly taking two phosphorylation sites of KaiC into account and we apply the extended model to the problem of synchrony of two oscillatory samples mixed at different phases. The agreement between the simulated and observed data suggests that the combined mechanism of the allosteric transition of KaiC hexamers and the monomer shuffling between them plays a key role in synchronization among KaiC hexamers and hence underlies the population-level oscillation of the ensemble of Kai proteins. The predicted synchronization patterns in mixtures of unequal amounts of two samples provide further opportunities to experimentally check the validity of the proposed mechanism. This mechanism of synchronization should be important in vivo for the persistent oscillation when Kai proteins are synthesized at random timing in cyanobacterial cells.
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