ArticleBiophysical journal2010
Massively parallel single-molecule manipulation using centrifugal force.
Article in Biophysical journal, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Protein force spectroscopy using magnetic tweezers: Slow and steady wins the race?Biophysical journal · 2026Review
- Massively parallel bead-free force spectroscopy with fluorescence.Science advances · 2026Article
- Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy.Annual review of biophysics · 2026Review
- Light-Guided Molecular Patterning for High-Throughput Single-Molecule Mechanical Characterization.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Modulating a Massive Set of Biomolecular Structures by Sono-Mechanical Force.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Investigating Polarity Effects in DNA Base Stacking.JACS Au · 2025Article
- Probing the Dynamic Strength of Biomolecular Interactions with Single-Cell Centrifugation.ACS central science · 2025Article
- A guide to building a low-cost centrifuge force microscope module for single-molecule force experiments.Nature protocols · 2025Review
- Tuning the Stability of DNA Tetrahedra with Base Stacking Interactions.Nano letters · 2025Article
- High-Throughput Centrifuge Force Microscopy Reveals Dynamic Immune-Cell Avidity at the Single-Cell Level.bioRxiv : the preprint server for biology · 2025Article
- Tuning the stability of DNA tetrahedra with base-stacking interactions.bioRxiv : the preprint server for biology · 2024Article
- Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide Resolution.Sensors (Basel, Switzerland) · 2023Article
- Combining DNA scaffolds and acoustic force spectroscopy to characterize individual protein bonds.Biophysical journal · 2023Article
- Resolving Molecular Heterogeneity with Single-Molecule Centrifugation.Journal of the American Chemical Society · 2023Article
- High-throughput single-molecule quantification of individual base stacking energies in nucleic acids.Nature communications · 2023Article
- Single-Molecule Mechanochemical Sensing.Accounts of chemical research · 2022Article
- A nanoscale DNA force spectrometer capable of applying tension and compression on biomolecules.Nucleic acids research · 2021Article
- Article
- Mechanical unfolding of ensemble biomolecular structures by shear force.Chemical science · 2021Article
- High-Throughput Test Paves the Way for Machine-Learning-Based Optimization of Adhesives.ACS central science · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Precise manipulation of single molecules has already led to remarkable insights in physics, chemistry, biology, and medicine. However, widespread adoption of single-molecule techniques has been impeded by equipment cost and the laborious nature of making measurements one molecule at a time. We have solved these issues by developing an approach that enables massively parallel single-molecule force measurements using centrifugal force. This approach is realized in an instrument that we call the centrifuge force microscope in which objects in an orbiting sample are subjected to a calibration-free, macroscopically uniform force-field while their micro-to-nanoscopic motions are observed. We demonstrate high-throughput single-molecule force spectroscopy with this technique by performing thousands of rupture experiments in parallel, characterizing force-dependent unbinding kinetics of an antibody-antigen pair in minutes rather than days. Additionally, we verify the force accuracy of the instrument by measuring the well-established DNA overstretching transition at 66 +/- 3 pN. With significant benefits in efficiency, cost, simplicity, and versatility, single-molecule centrifugation has the potential to expand single-molecule experimentation to a wider range of researchers and experimental systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.