ArticleCurrent microbiology2011
Simultaneous amplification of two bacterial genes: more reliable method of Helicobacter pylori detection in microbial rich dental plaque samples.
Article in Current microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Oral urease rapid test serve as an effective complement to the detection of Helicobacter pylori infection through urea breath test.BMC gastroenterology · 2025Article
- Multiplex Accelerated PCR System for One-Step Helicobacter pylori cagA + Genotypes Detection: A Guide for Clinical Testing.Current microbiology · 2022Article
- Are the view of Helicobacter pylori colonized in the oral cavity an illusion?Experimental & molecular medicine · 2017Review
- The investigation of Helicobacter pylori in the dental biofilm and saliva samples of children with dyspeptic complaints.BMC oral health · 2017Article
- Occurrence ofGastroenterology and hepatology from bed to bench · 2017Article
- Helicobacter pylori identification: a diagnostic/confirmatory method for evaluation.Current microbiology · 2014Article
- Helicobacter pylori and oral pathology: relationship with the gastric infection.World journal of gastroenterology · 2014Article
- Helicobacter pylori infection - recent developments in diagnosis.World journal of gastroenterology · 2014Review
- Role of dental plaque, saliva and periodontal disease in Helicobacter pylori infection.World journal of gastroenterology · 2014Review
- An association between Helicobacter pylori and upper respiratory tract disease: fact or fiction?World journal of gastroenterology · 2014Review
- Oral Cavity as an Extragastric Reservoir of Helicobacter pylori.ISRN gastroenterology · 2014Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polymerase Chain reaction (PCR) assay is considered superior to other methods for detection of Helicobacter pylori (H. pylori) in oral cavity; however, it also has limitations when sample under study is microbial rich dental plaque. The type of gene targeted and number of primers used for bacterial detection in dental plaque samples can have a significant effect on the results obtained as there are a number of closely related bacterial species residing in plaque biofilm. Also due to high recombination rate of H. pylori some of the genes might be down regulated or absent. The present study was conducted to determine the frequency of H. pylori colonization of dental plaque by simultaneously amplifying two genes of the bacterium. One hundred dental plaque specimens were collected from dyspeptic patients before their upper gastrointestinal endoscopy and presence of H. pylori was determined through PCR assay using primers targeting two different genes of the bacterium. Eighty-nine of the 100 samples were included in final analysis. With simultaneous amplification of two bacterial genes 51.6% of the dental plaque samples were positive for H. pylori while this prevalence increased to 73% when only one gene amplification was used for bacterial identification. Detection of H. pylori in dental plaque samples is more reliable when two genes of the bacterium are simultaneously amplified as compared to one gene amplification only.
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