ArticleJournal of assisted reproduction and genetics2010
Slow and ultrarapid cryopreservation of biopsied mouse blastocysts and its effect on DNA integrity index.
Article in Journal of assisted reproduction and genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
6 citing papers in PubMed.
- The effect of coenzyme Q10 on cryotolerance of in vivo-derived mouse embryos.JBRA assisted reproduction · 2024Article
- Prolonged Cryopreservation Negatively Affects Embryo Transfer Outcomes Following the Elective Freeze-All Strategy: A Multicenter Retrospective Study.Frontiers in endocrinology · 2021Article
- The profiling of pre- and post-warming DNA in mouse embryos with microsatellite method.Veterinary world · 2018Article
- Subzero organ preservation: the dawn of a new ice age?Current opinion in organ transplantation · 2017Review
- Different chromatin and energy/redox responses of mouse morulae and blastocysts to slow freezing and vitrification.Reproductive biology and endocrinology : RB&E · 2015Article
- Slow freezing and vitrification of mouse morula and early blastocysts.Journal of assisted reproduction and genetics · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo evaluate the effect of slow and ultra-rapid freezing on biopsied blastocysts' DNA integrity.
methodsForty eight mouse blastocysts were biopsied of which 16 were cryopreserved by slowly freezing and 17 by vitrification. Fourteen intact blastocysts were slowly cryopreserved and 24 were vitrified. Eighteen fresh intact blastocysts and fifteen biopsied blastocysts served as controls. The DNA integrity index of all blastocysts was evaluated using (TUNEL) staining and confocal imaging
resultsBoth slow freezing and vitrification of biopsied blastocysts induced apoptosis to a similar extent. Biopsying blastocysts before vitrification resulted in less apoptosis than vitrification of intact blastocysts.
conclusionSlow freezing and vitrification are equal options for preservation of biopsied blastocysts as regards the DNA integrity index (DII). Biopsied blastocysts better tolerate vitrification than intact expanded blastocysts.
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Registered trials
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