ArticleNature methods2010
Probabilistic density maps to study global endomembrane organization.
Article in Nature methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 100 citations in OpenAlex.
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- Peripheral positioning of lysosomes supports melanoma aggressiveness.Nature communications · 2025Article
- Trafficking in cancer: from gene deregulation to altered organelles and emerging biophysical properties.Frontiers in cell and developmental biology · 2024Review
- Quantifying spatial dynamics of Mycobacterium tuberculosis infection of human macrophages using microfabricated patterns.Cell reports methods · 2023Article
- Cellular harmonics for the morphology-invariant analysis of molecular organization at the cell surface.Nature computational science · 2023Article
- Spatial organization of lysosomal exocytosis relies on membrane tension gradients.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Transcription factor EB regulates phosphatidylinositol-3-phosphate levels that control lysosome positioning in the bladder cancer model.Communications biology · 2023Article
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- Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking.eLife · 2022Article
- Autophagy Is Polarized toward Cell Front during Migration and Spatially Perturbed by Oncogenic Ras.Cells · 2021Article
- Interrogating RNA and protein spatial subcellular distribution in smFISH data with DypFISH.Cell reports methods · 2021Article
- Cell shape regulates subcellular organelle location to control early CaScientific reports · 2020Article
- Endomembranes: Unsung Heroes of Mechanobiology?Frontiers in bioengineering and biotechnology · 2020Review
- Actomyosin-driven force patterning controls endocytosis at the immune synapse.Nature communications · 2019Article
- Article
- Quantifying exosome secretion from single cells reveals a modulatory role for GPCR signaling.The Journal of cell biology · 2018Article
- High resolution microscopy reveals the nuclear shape of budding yeast during cell cycle and in various biological states.Journal of cell science · 2016Article
- Mapping intracellular mechanics on micropatterned substrates.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a computational imaging approach that describes the three-dimensional spatial organization of endomembranes from micromanipulation-normalized mammalian cells with probabilistic density maps. Applied to several well-known marker proteins, this approach revealed the average steady-state organization of early endosomes, multivesicular bodies or lysosomes, endoplasmic reticulum exit sites, the Golgi apparatus and Golgi-derived transport carriers in crossbow-shaped cells. The steady-state organization of each tested endomembranous population was well-defined, unique and in some cases depended on the cellular adhesion geometry. Density maps of all endomembrane populations became stable when pooling several tens of cells only and were reproducible in independent experiments, allowing construction of a standardized cell model. We detected subtle changes in steady-state organization induced by disruption of the cellular cytoskeleton, with statistical significance observed for just 20 cells. Thus, combining micropatterning with construction of endomembrane density maps allows the systematic study of intracellular trafficking determinants.
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