ArticleNature biotechnology2010
Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511.
Article in Nature biotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 251 papers.
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Who cites it
251 citing papers in PubMed, 556 citations in OpenAlex.
- Human Hematopoietic Stem Cells Enhance Maturational Differentiation of hiPSC-Derived Cardiomyocytes on Xeno-Free MatriClone-Plastic via EGFR/MAPK/ERK Signaling Pathway.Pharmaceuticals (Basel, Switzerland) · 2026Article
- Roles and therapeutic prospects of the laminin family in disorders of the nervous system.Journal of translational medicine · 2026Review
- Collagen IV outperforms alternative ECM coatings to preserve human neural progenitor properties on electrically conductive neural interfaces.Scientific reports · 2026Article
- Vascular basement membrane laminins modulate functional zonation of cerebral microvessels.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026Article
- Adjusting PSC culture for neural organoid generation.Stem cell reports · 2026Article
- NN15-017 promotes human pluripotent stem cell proliferation and generation.Regenerative therapy · 2025Article
- Advances in Induced Pluripotent Stem Cell Reprogramming and Its Application in Amyotrophic Lateral Sclerosis: A Review.FASEB bioAdvances · 2025Review
- Animal-free alternatives for Matrigel in human iPSC-derived blood vessel organoid culture.Scientific reports · 2025Article
- Validated methods for isolation and qualification of mesenchymal stromal/stem cells from different sources.Journal of translational medicine · 2025Review
- Retina-specific laminin-based generation of photoreceptor progenitors from human pluripotent stem cells under xeno-free and chemically defined conditions.Nature protocols · 2025Review
- Large-Scale Bioprinting of Human Epiblast-Like Models Featuring Disc-Shaped Morphogenesis and Gastrulation Events.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Chemically defined and xeno-free media enables the derivation of human extended pluripotent stem cell lines from discarded blastocysts with a high efficiency.Cell & bioscience · 2025Article
- Profiling human iPSC-derived sensory neurons for analgesic drug screening using a multi-electrode array.Cell reports methods · 2025Article
- Supramolecular Additive Screening to Engineer Microfibrous Rafts for Expansion of Pluripotent Stem Cells in Dynamic Suspension.Advanced healthcare materials · 2025Article
- Advancements in theFrontiers in toxicology · 2025Review
- Differentiation of human induced pluripotent stem cells into retinal pigment epithelium cells during culture on peptide-grafted hydrogels.Regenerative biomaterials · 2025Article
- The Past, Present, and Future Development of Cell Culture Substrates for PSC-Derived Therapies and the Challenges in Meeting the Plethora of Emerging User Requirements.Advances in experimental medicine and biology · 2025Review
- Poly(norepinephrine)-Mediated Universal Surface Modification for Patterning Human Pluripotent Stem Cell Culture and Differentiation.ACS biomaterials science & engineering · 2024Article
- Heparan Sulfate Chain-Conjugated Laminin-E8 Fragments Advance Paraxial Mesodermal Differentiation Followed by High Myogenic Induction from hiPSCs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Laminin 511 E8 fragment promotes to form basement membrane-like structure in human skin equivalents.Regenerative therapy · 2024Article
191 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a system for culturing human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells on a recombinant form of human laminin-511, a component of the natural hES cell niche. The system is devoid of animal products and feeder cells and contains only one undefined component, human albumin. The hES cells self-renewed with normal karyotype for at least 4 months (20 passages), after which the cells could produce teratomas containing cell lineages of all three germ layers. When plated on laminin-511 in small clumps, hES cells spread out in a monolayer, maintaining cellular homogeneity with approximately 97% OCT4-positive cells. Adhesion of hES cells was dependent on alpha6beta1 integrin. The use of homogeneous monolayer hES or iPS cell cultures provides more controllable conditions for the design of differentiation methods. This xeno-free and feeder-free system may be useful for the development of cell lineages for therapeutic purposes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.